气候变化领域集成服务门户(CCPortal): An efficient and gentle enzymatic digestion protocol for the extraction of microplastics from bivalve tissue

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DOI	10.1016/j.marpolbul.2019.03.016
	An efficient and gentle enzymatic digestion protocol for the extraction of microplastics from bivalve tissue
	von Friesen L.W.; Granberg M.E.; Hassellöv M.; Gabrielsen G.W.; Magnusson K.
发表日期	2019
ISSN	0025326X
起始页码	129
结束页码	134
卷号	142
英文摘要	Standardized methods for the digestion of biota for microplastic analysis are currently lacking. Chemical methods can be effective, but can also cause damage to some polymers. Enzymatic methods are known to be gentler, but often laborious, expensive and time consuming. A novel tissue digestion method with pancreatic enzymes and a pH buffer (Tris) is here presented in a comparison to a commonly applied digestion protocol with potassium hydroxide. The novel protocol demonstrates a highly efficient removal of bivalve tissue (97.7 ± 0.2% dry weight loss) already over-night. Furthermore, it induces no impairment in terms of ability to correctly identify four pre-weathered plastic polymers and six textile fiber polymers by Fourier transform infrared spectroscopy after exposure. The high-throughput protocol requires minimal handling, is of low cost and does not pose risk to the performer or the environment. It is therefore suggested as a candidate for a standardized digestion protocol, enabling successful analysis of microplastics ingested by bivalves. © 2019 Elsevier Ltd
英文关键词	Emerging pollutants; Enzymatic digestion protocol; KOH; Microplastics; Pancreatic enzymes; Tissue digestion
语种	英语
scopus关键词	Enzymes; Fourier transform infrared spectroscopy; Microplastic; Molluscs; Potassium hydroxide; Emerging pollutants; Enzymatic digestions; Enzymatic methods; High-throughput protocols; Microplastics; Plastic polymers; Standardized methods; Tissue digestions; Tissue; microplastic; pancreas enzyme; plastic; polymer; unclassified drug; buffer; enzyme; hydroxide; plastic; potassium derivative; potassium hydroxide; bioaccumulation; bivalve; digestion; enzyme; extraction method; nanoparticle; pH; plastic; polymer; animal tissue; Article; body weight loss; clinical protocol; controlled study; cost; environment; enzyme assisted extraction; exposure variable; extraction and extracts; Fourier transform infrared spectroscopy; high throughput screening; nonhuman; standardization; analysis; animal; bivalve; chemistry; comparative study; ecotoxicology; environmental monitoring; infrared spectroscopy; isolation and purification; pH; procedures; textile; water pollutant; Bivalvia; Animals; Bivalvia; Buffers; Ecotoxicology; Environmental Monitoring; Enzymes; Hydrogen-Ion Concentration; Hydroxides; Plastics; Potassium Compounds; Spectroscopy, Fourier Transform Infrared; Textiles; Water Pollutants, Chemical
来源期刊	Marine Pollution Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/149966
作者单位	IVL, Swedish Environmental Research Institute, Kristineberg 566, Fiskebäckskil, SE-45178, Sweden; University of Gothenburg, Department of Marine Sciences, Kristineberg 566, Fiskebäckskil, SE-45178, Sweden; Norwegian Polar Institute, Hjalmar Johansensgate 14, Tromsø, N-9296, Norway
推荐引用方式 GB/T 7714	von Friesen L.W.,Granberg M.E.,Hassellöv M.,et al. An efficient and gentle enzymatic digestion protocol for the extraction of microplastics from bivalve tissue[J],2019,142.
APA	von Friesen L.W.,Granberg M.E.,Hassellöv M.,Gabrielsen G.W.,&Magnusson K..(2019).An efficient and gentle enzymatic digestion protocol for the extraction of microplastics from bivalve tissue.Marine Pollution Bulletin,142.
MLA	von Friesen L.W.,et al."An efficient and gentle enzymatic digestion protocol for the extraction of microplastics from bivalve tissue".Marine Pollution Bulletin 142(2019).