气候变化领域集成服务门户(CCPortal): Studies on diversity of Vibrio sp. and the prevalence of hapA; tcpI; st; rtxA&C; acfB; hlyA; ctxA; ompU and toxR genes in environmental strains of Vibrio cholerae from Port Blair bays of South Andaman; India

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DOI	10.1016/j.marpolbul.2019.05.011
	Studies on diversity of Vibrio sp. and the prevalence of hapA; tcpI; st; rtxA&C; acfB; hlyA; ctxA; ompU and toxR genes in environmental strains of Vibrio cholerae from Port Blair bays of South Andaman; India
	Meena B.; Anburajan L.; Sathish T.; Das A.K.; Vinithkumar N.V.; Kirubagaran R.; Dharani G.
发表日期	2019
ISSN	0025326X
起始页码	105
结束页码	116
卷号	144
英文摘要	Vibrio species are widely distributed in the estuarine and coastal waters that possess the greatest threat to human health worldwide. In this study it is aimed to isolate and observe the abundance of Vibrio sp. and prevalence of biomarker genes and antibiotic resistance profile of V. cholerae isolated from the Port Blair bays of South Andaman. A total of 56 water samples were collected from the seven sampling stations of Port Blair bays in which maximum number of Vibrio sp. population density (1.78 × 104) was recorded in Phoenix Bay. Among the 786 isolates 57.38% of the isolates were confirmed as Vibrio sp., Vibrio cholerae and Vibrio parahaemolyticus. PCR results revealed that the prevalence of biomarker genes was recorded maximum in the isolates from Phoenix Bay and Junglighat Bay samples. Upon further analysis, it was observed that the prevalence of hlyA gene (215 bp), was found to be the most widespread biomarker determinant in 84.17% of isolates. Major virulence determinants; ctxA, ompU and toxR genes were not detected in V. cholerae isolates from Port Blair bays. Maximum antibiotic resistance pattern was observed in Phoenix Bay isolates and maximum number of V. cholerae isolates was resistance to tetracycline (60.76%). Cluster and Principal Component Analysis were employed to understand the diversity and distribution of Vibrio isolates and its biomarker genes. Upon PCA analysis seasonal influence was not much perceived in Vibrio species diversity in Port Blair bays and the lack of significant difference in the detection of species diversity in this study is due to resemblance in geographical conditions and sources of pollution. © 2019 Elsevier Ltd
英文关键词	Antibiotic resistance; Biomarker genes; Cluster analysis; Principal Component Analysis; Vibrio sp.
语种	英语
scopus关键词	Antibiotics; Biodiversity; Biomarkers; Cluster analysis; Genes; Health risks; Population statistics; Antibiotic resistance; Environmental strain; Geographical conditions; Population densities; Sampling stations; Vibrio cholerae; Vibrio parahaemolyticus; Vibrio sp; Principal component analysis; tetracycline; virulence factor; abundance; antibiotic resistance; bacterium; biomarker; cluster analysis; gene; population density; principal component analysis; species diversity; acfB gene; antibiotic resistance; Article; bacterial gene; bacterial strain; bacterial virulence; bacterium isolation; controlled study; ctxA gene; gene sequence; genetic variability; hapA gene; hlyA gene; India; molecular cloning; neighbor joining method; nonhuman; nucleotide sequence; ompU gene; physical chemistry; polymerase chain reaction; population density; prevalence; rtxA gene; rtxC gene; st gene; tcpI gene; toxR gene; Vibrio cholerae; Vibrio parahaemolyticus; water sampling; bay; biodiversity; genetics; human; isolation and purification; microbiology; species difference; Vibrio cholerae; Andamans; Andamans and Nicobars; India; Port Blair Bay; South Andaman; Vibrio; Vibrio cholerae; Vibrio parahaemolyticus; Vibrio sp.; Bays; Biodiversity; Drug Resistance, Bacterial; Genes, Bacterial; Humans; India; Prevalence; Species Specificity; Vibrio cholerae; Vibrio parahaemolyticus; Virulence Factors
来源期刊	Marine Pollution Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/149860
作者单位	Atal Centre for Ocean Science and Technology for Islands, National Institute of Ocean Technology, Port Blair, Andaman and Nicobar Islands 744103, India; Marine Biotechnology Division, Ocean Science and Technology for Islands Group, National Institute of Ocean Technology, Ministry of Earth Sciences, Government of India, Chennai, Tamil Nadu 600100, India
推荐引用方式 GB/T 7714	Meena B.,Anburajan L.,Sathish T.,et al. Studies on diversity of Vibrio sp. and the prevalence of hapA; tcpI; st; rtxA&C; acfB; hlyA; ctxA; ompU and toxR genes in environmental strains of Vibrio cholerae from Port Blair bays of South Andaman; India[J],2019,144.
APA	Meena B..,Anburajan L..,Sathish T..,Das A.K..,Vinithkumar N.V..,...&Dharani G..(2019).Studies on diversity of Vibrio sp. and the prevalence of hapA; tcpI; st; rtxA&C; acfB; hlyA; ctxA; ompU and toxR genes in environmental strains of Vibrio cholerae from Port Blair bays of South Andaman; India.Marine Pollution Bulletin,144.
MLA	Meena B.,et al."Studies on diversity of Vibrio sp. and the prevalence of hapA; tcpI; st; rtxA&C; acfB; hlyA; ctxA; ompU and toxR genes in environmental strains of Vibrio cholerae from Port Blair bays of South Andaman; India".Marine Pollution Bulletin 144(2019).