气候变化领域集成服务门户(CCPortal): Biodegradation of crude oil using self-immobilized hydrocarbonoclastic deep sea bacterial consortium

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DOI	10.1016/j.marpolbul.2019.07.006
	Biodegradation of crude oil using self-immobilized hydrocarbonoclastic deep sea bacterial consortium
	Ganesh Kumar A.; Nivedha Rajan N.; Kirubagaran R.; Dharani G.
发表日期	2019
ISSN	0025326X
起始页码	741
结束页码	750
卷号	146
英文摘要	Hydrocarbonoclastic bacterial consortium that utilizes crude oil as carbon and energy source was isolated from marine sediment collected at a depth of 2100 m. Molecular characterization by 16S rRNA gene sequences confirmed that these isolates as Oceanobacillus sp., Nesiotobacter sp., Ruegeria sp., Photobacterium sp., Enterobacter sp., Haererehalobacter sp., Exiguobacterium sp., Acinetobacter sp. and Pseudoalteromonas sp. Self-immobilized consortium degraded more than 85% of total hydrocarbons after 10 days of incubation with 1% (v/v) of crude oil and 0.05% (v/v) of Tween 80 (non-ionic surfactant) at 28 ± 2 °C. The addition of nitrogen and phosphorus sources separately i.e. 0.1% (v/v) of CO (NH2)2 or K2HPO4 enhanced the hydrocarbon utilization percentage. The pathways of microbial degradation of hydrocarbons were confirmed by FTIR, GC–MS, 1H and 13C NMR spectroscopy analyses. These results demonstrated a novel approach using hydrocarbonoclastic self-immobilized deep sea bacterial consortium for eco-friendly bioremediation. © 2019
英文关键词	Biodegradation; Bioremediation; Hydrocarbon; Hydrocarbonoclastic; Self-immobilization
语种	英语
scopus关键词	Bioremediation; Biotechnology; Crude oil; Fourier transform infrared spectroscopy; Hydrocarbons; Nuclear magnetic resonance spectroscopy; RNA; Submarine geology; 13C NMR spectroscopy; 16S rRNA gene sequence; Hydrocarbonoclastic; Microbial degradation; Molecular characterization; Nitrogen and phosphorus; Pseudoalteromonas sp; Self-immobilization; Biodegradation; hydrocarbon; nitrogen; petroleum; phosphorus; polysorbate 80; RNA 16S; hydrocarbon; petroleum; RNA 16S; sea water; bacterium; biodegradation; bioremediation; crude oil; deep sea; hydrocarbon; immobilization; Acinetobacter; Article; biodegradation; bioremediation; carbon nuclear magnetic resonance; carbon utilization; deep sea; Enterobacter; Exiguobacterium; Fourier transform infrared spectroscopy; gene sequence; Haererehalobacter; immobilization; incubation time; marine bacterium; mass fragmentography; microbial degradation; Nesiotobacter; nonhuman; Oceanobacillus; Photobacterium; proton nuclear magnetic resonance; Pseudoalteromonas; Ruegeria; sediment; bioremediation; dietary fiber; genetics; immobilized cell; Indian Ocean; infrared spectroscopy; metabolism; microbial consortium; microbiology; nuclear magnetic resonance spectroscopy; physiology; Rhodobacteraceae; Acinetobacter sp.; Bacteria (microorganisms); Enterobacter sp.; Exiguobacterium sp.; Haererehalobacter; Oceanobacillus; Photobacterium sp.; Pseudoalteromonas sp.; Ruegeria; Acinetobacter; Biodegradation, Environmental; Cells, Immobilized; Dietary Fiber; Gas Chromatography-Mass Spectrometry; Geologic Sediments; Hydrocarbons; Indian Ocean; Magnetic Resonance Spectroscopy; Microbial Consortia; Nitrogen; Petroleum; Phosphorus; Pseudoalteromonas; Rhodobacteraceae; RNA, Ribosomal, 16S; Seawater; Spectroscopy, Fourier Transform Infrared
来源期刊	Marine Pollution Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/149681
作者单位	Marine Biotechnology Division, Earth System Science Organization - National Institute of Ocean Technology (ESSO – NIOT), Ministry of Earth Sciences (MoES), Government of India, Pallikaranai, Chennai, 600100, India
推荐引用方式 GB/T 7714	Ganesh Kumar A.,Nivedha Rajan N.,Kirubagaran R.,et al. Biodegradation of crude oil using self-immobilized hydrocarbonoclastic deep sea bacterial consortium[J],2019,146.
APA	Ganesh Kumar A.,Nivedha Rajan N.,Kirubagaran R.,&Dharani G..(2019).Biodegradation of crude oil using self-immobilized hydrocarbonoclastic deep sea bacterial consortium.Marine Pollution Bulletin,146.
MLA	Ganesh Kumar A.,et al."Biodegradation of crude oil using self-immobilized hydrocarbonoclastic deep sea bacterial consortium".Marine Pollution Bulletin 146(2019).