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| DOI | 10.1016/j.marpolbul.2019.07.050 |
| Development and validation of eDNA markers for the detection of Crepidula fornicata in environmental samples | |
| Miralles L.; Parrondo M.; Hernández de Rojas A.; Garcia-Vazquez E.; Borrell Y.J. | |
| 发表日期 | 2019 |
| ISSN | 0025326X |
| 起始页码 | 827 |
| 结束页码 | 830 |
| 卷号 | 146 |
| 英文摘要 | The invasive Crepidula fornicata caused major problems along the European Atlantic coast, especially in France and Netherlands where high densities leads on changes in the habitat, disturb native marine wildlife as well as it originates competition for space and food. Despite its dangerous invasive nature, regular monitoring to alert about its presence in risk areas, like the south Bay of Biscay (Spain and south France), is not done yet. Here, we developed a species-specific marker to detect the presence of C. fornicata in environmental samples (eDNA) of seawater. The novel C. fornicata specific primers amplified a region of 239 bp within the COI gen. We employed this tool to check its presence in 6 estuaries of the Cantabrian Sea, an area comprised between the Spanish and French limits of the previously reported presence of this limpet in the south Bay of Biscay. The presence of C. fornicata was confirmed in A Coruña (Galicia, Spain), Eo and Villaviciosa estuaries (Asturias, Spain) while it was not detected in Santander, Bilbao (Spain), and Bayonne (France). This new method to detect C. fornicata could be easily implemented in regular monitoring to prevent and manage future invasions of this species. © 2019 Elsevier Ltd |
| 英文关键词 | Bay of Biscay; Crepidula fornicata; Detection; eDNA; Invasive species; Specific primers |
| 语种 | 英语 |
| scopus关键词 | Contamination; Error detection; Marine pollution; Bay of Biscay; Crepidula fornicata; eDNA; Invasive species; Specific primers; Estuaries; DNA; environmental DNA; sea water; unclassified drug; primer DNA; clam culture; competition (ecology); detection method; DNA; invasive species; marine pollution; pollution effect; Article; controlled study; Crepidula fornicata; DNA determination; environmental factor; environmental management; environmental monitoring; estuary; France; genetic marker; nonhuman; Spain; species difference; validation study; animal; ecosystem; gastropod; genetics; introduced species; Netherlands; polymerase chain reaction; procedures; Atlantic Ocean; Bay of Biscay; France; Netherlands; Spain; Crepidula fornicata; Animals; DNA Primers; Ecosystem; Environmental Monitoring; France; Gastropoda; Genetic Markers; Introduced Species; Netherlands; Polymerase Chain Reaction; Seawater; Spain; Species Specificity |
| 来源期刊 | Marine Pollution Bulletin
 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/149657 |
| 作者单位 | Department of Functional Biology, Genetics, University of Oviedo, C. Julián Clavería s/n, Oviedo, 33006, Spain; Spanish Institute of Oceanography, Oceanographic Center of Gijón, Avda. Príncipe de Asturias, 70 bis, Gijón, 33212, Spain |
| 推荐引用方式 GB/T 7714 | Miralles L.,Parrondo M.,Hernández de Rojas A.,et al. Development and validation of eDNA markers for the detection of Crepidula fornicata in environmental samples[J],2019,146. |
| APA | Miralles L.,Parrondo M.,Hernández de Rojas A.,Garcia-Vazquez E.,&Borrell Y.J..(2019).Development and validation of eDNA markers for the detection of Crepidula fornicata in environmental samples.Marine Pollution Bulletin,146. |
| MLA | Miralles L.,et al."Development and validation of eDNA markers for the detection of Crepidula fornicata in environmental samples".Marine Pollution Bulletin 146(2019). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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