气候变化领域集成服务门户(CCPortal): Incubation in light versus dark affects the vitality of UV-irradiated Tetraselmis suecica differently: A flow cytometric study

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DOI	10.1016/j.marpolbul.2019.110528
	Incubation in light versus dark affects the vitality of UV-irradiated Tetraselmis suecica differently: A flow cytometric study
	Olsen R.O.; Lindivat M.; Larsen A.; Thuestad G.; Hoell I.A.
发表日期	2019
ISSN	0025326X
卷号	149
英文摘要	In this study, we used flow cytometry to examine how incubation in dark versus light affects the vitality and viability of UV-irradiated Tetraselmis suecica. High UV doses (300 and 400 mJ/cm2) affected the esterase activity, membrane permeability, and chlorophyll content more when the subsequent incubation took place in light. For non- or low UV dose (100 and 200 mJ/cm2)-treated cells, incubation in light resulted in cell regrowth as compared to incubation in dark. Damaged cells (enzymatically active but with permeable membranes) did not recover when incubated under light or dark conditions. Exposure to light reduces the evaluation time of any given ballast water treatment, as viable cells will be detected at an earlier stage and the vitality is more affected. When evaluating the performance of UV-based ballast water treatment systems (BWTS), these results can be useful for type approval using T. suecica as a test organism in the test regime. © 2019 Elsevier Ltd
英文关键词	Ballast water; Flow cytometry; Incubation; Phytoplankton; Vitality
语种	英语
scopus关键词	Ballast (railroad track); Cells; Cytology; Flow cytometry; Phytoplankton; Water; Ballast water; Ballast water treatment; Ballast water treatment system; Chlorophyll contents; Incubation; Membrane permeability; Permeable membranes; Vitality; Water treatment; ballast water; chlorophyll; esterase; unclassified drug; water; 5-carboxyfluorescein diacetate acetoxymethyl ester; chlorophyll; esterase; fluorescein derivative; ballast water; experimental study; flow cytometry; growth response; incubation; light effect; numerical model; phytoplankton; ultraviolet radiation; Article; cell damage; cell growth; cell viability; chlorophyll content; controlled study; darkness; enzyme activity; flow cytometry; incubation; light; membrane permeability; nonhuman; procedures concerning cells; radiation dose; Tetraselmis; Tetraselmis suecica; ultraviolet irradiation; water treatment; darkness; green alga; light; metabolism; physiology; phytoplankton; procedures; radiation response; ultraviolet radiation; water management; Tetraselmis suecica; Chlorophyll; Chlorophyta; Darkness; Dose-Response Relationship, Radiation; Esterases; Flow Cytometry; Fluoresceins; Light; Phytoplankton; Ultraviolet Rays; Water Purification
来源期刊	Marine Pollution Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/149564
作者单位	Western Norway University of Applied Science, Bjoernsonsgt. 45, Haugesund, 5528, Norway; Uni Research Environment and Hjort Centre for Marine Ecosystem Dynamics, Bergen, 5006, Norway
推荐引用方式 GB/T 7714	Olsen R.O.,Lindivat M.,Larsen A.,et al. Incubation in light versus dark affects the vitality of UV-irradiated Tetraselmis suecica differently: A flow cytometric study[J],2019,149.
APA	Olsen R.O.,Lindivat M.,Larsen A.,Thuestad G.,&Hoell I.A..(2019).Incubation in light versus dark affects the vitality of UV-irradiated Tetraselmis suecica differently: A flow cytometric study.Marine Pollution Bulletin,149.
MLA	Olsen R.O.,et al."Incubation in light versus dark affects the vitality of UV-irradiated Tetraselmis suecica differently: A flow cytometric study".Marine Pollution Bulletin 149(2019).