气候变化领域集成服务门户(CCPortal): Application of a rapid qPCR method for enterococci for beach water quality monitoring purposes in Hawaii: Loss of DNA during the extraction protocol due to coral sands

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DOI	10.1016/j.marpolbul.2019.110631
	Application of a rapid qPCR method for enterococci for beach water quality monitoring purposes in Hawaii: Loss of DNA during the extraction protocol due to coral sands
	Seruge J.; Wong M.; Noble R.T.; Blackwood A.D.; Moravcik P.S.; Kirs M.
发表日期	2019
ISSN	0025326X
卷号	149
英文摘要	Rapid qPCR methods for enumerating enterococci can provide results in a few hours, thereby enhancing public health protection. Analysis of 140 samples collected from 11 beaches in Hawaii for enterococci using EPA Method 1611 revealed that a majority (70%) of samples yielded unusable data using the recommended protocol due to DNA losses during extraction. The DNA loss was correlated to the presence of suspended coralline sand. Acidification of samples alleviated or removed the interference, enabling successful method application. There were significant correlations across the three methods evaluated (Enterolert®/Method 1600 R2 = 0.85, Enterolert®/ Method 1611 R2 = 0.78 and Method 1600/1611 R2 = 0.82). When samples were not compromised, there was also good agreement among methods for beach management decisions. This study presents a protocol for beach areas with coralline sands, and re-emphasizes the need to use appropriate controls to prevent underestimation of bacterial concentrations at recreational beaches. © 2019 Elsevier Ltd
英文关键词	Coastal water quality; Enterococci; EPA method 1611; Hawaii; Interference
语种	英语
scopus关键词	Beaches; DNA; Environmental Protection Agency; Polymerase chain reaction; Water quality; Wave interference; Beach water qualities; Coastal water quality; Enterococci; EPA method; Extraction protocols; Hawaii; Public health protection; Recreational beaches; Extraction; bacterium; coastal water; DNA; extraction method; monitoring; polymerase chain reaction; sand; water quality; acidification; article; coastal waters; coral; extraction; Hawaii; intermethod comparison; major clinical study; seashore; water quality; Hawaii [United States]; United States; Anthozoa; Bacteria (microorganisms)
来源期刊	Marine Pollution Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/149471
作者单位	Water Resources Research Center, University of Hawaii, 2540 Dole Street, Holmes Hall 283, Honolulu, HI 96822, United States; Institute of Marine Sciences, University of North Carolina at Chapel Hill, 3431 Arendell Street, Morehead City, NC 28557, United States
推荐引用方式 GB/T 7714	Seruge J.,Wong M.,Noble R.T.,et al. Application of a rapid qPCR method for enterococci for beach water quality monitoring purposes in Hawaii: Loss of DNA during the extraction protocol due to coral sands[J],2019,149.
APA	Seruge J.,Wong M.,Noble R.T.,Blackwood A.D.,Moravcik P.S.,&Kirs M..(2019).Application of a rapid qPCR method for enterococci for beach water quality monitoring purposes in Hawaii: Loss of DNA during the extraction protocol due to coral sands.Marine Pollution Bulletin,149.
MLA	Seruge J.,et al."Application of a rapid qPCR method for enterococci for beach water quality monitoring purposes in Hawaii: Loss of DNA during the extraction protocol due to coral sands".Marine Pollution Bulletin 149(2019).