气候变化领域集成服务门户(CCPortal): Genotoxic biomarkers and histological changes in marine medaka (Oryzias melastigma) exposed to 17α-ethynylestradiol and 17β-trenbolone

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DOI	10.1016/j.marpolbul.2019.110601
	Genotoxic biomarkers and histological changes in marine medaka (Oryzias melastigma) exposed to 17α-ethynylestradiol and 17β-trenbolone
	Zhang Y.; Wang J.; Lu L.; Li Y.; Wei Y.; Cheng Y.; Zhang X.; Tian H.; Wang W.; Ru S.
发表日期	2020
ISSN	0025326X
卷号	150
英文摘要	Endocrine-disrupting pollutants in marine environments have aroused great concern for their adverse effects on the reproduction of marine organisms. This study aimed to seek promising biomarkers for estrogenic/androgenic chemicals. First, two possible male-specific genes, SRY-box containing gene 9a2 (sox9a2) and gonadal soma-derived factor (gsdf), were cloned from marine medaka (Oryzias melastigma). Then the responses of sox9a2, gsdf, choriogenin (chgH and chgL), vitellogenin (vtg1 and vtg2), and cytochrome P450 aromatase (cyp19a and cyp19b) were investigated after exposure to 17α-ethynylestradiol (EE2) and 17β-trenbolone (TB) at 2, 10, and 50 ng/L. The results showed that gsdf was specifically expressed in the testes and easily induced in the ovaries after TB exposure, indicating that gsdf was a potential biomarker of environmental androgens. ChgL was a useful biomarker of weak estrogen pollution for its high sensitivity to low levels of EE2. In addition, both EE2 and TB exposure damaged gonadal structures and inhibited gonadal development. © 2019 Elsevier Ltd
英文关键词	17α-ethynylestradiol; 17β-trenbolone; Biomarker; Endocrine disruptor; Gonadal soma-derived factor (gsdf); Marine medaka
语种	英语
scopus关键词	Cell proliferation; Cloning; Endocrine disrupters; Genes; Marine biology; Marine pollution; Endocrine disruptor; Ethynylestradiol; Gonadal soma-derived factor (gsdf); Marine medaka; Trenbolone; Biomarkers; aromatase; ethinylestradiol; trenbolone; biological marker; ethinylestradiol; trenbolone acetate; androgen; biomarker; endocrine disruptor; estrogenic compound; finfish; genotoxicity; histology; immune response; pollution exposure; reproduction; adult; animal cell; animal experiment; animal model; animal tissue; choriogenin H gene; choriogenin L gene; controlled study; cyp19a gene; cyp19b gene; environmental exposure; female; gene; gene expression; genotoxicity; gonad development; gonadal disease; gonadal soma derived factor gene; histology; male; nonhuman; Oryzias melastigma; ovary; sea pollution; SRY box containing gene 9a2; testis tissue; vitellogenin 1 gene; vitellogenin 2 gene; animal; DNA damage; Oryzias; physiology; toxicity; water pollutant; Oryzias melastigma; Oryziinae; Animals; Biomarkers; DNA Damage; Ethinyl Estradiol; Female; Male; Oryzias; Trenbolone Acetate; Water Pollutants, Chemical
来源期刊	Marine Pollution Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/149432
作者单位	College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China; School of Public Health, Qingdao University, Qingdao, 266021, China
推荐引用方式 GB/T 7714	Zhang Y.,Wang J.,Lu L.,et al. Genotoxic biomarkers and histological changes in marine medaka (Oryzias melastigma) exposed to 17α-ethynylestradiol and 17β-trenbolone[J],2020,150.
APA	Zhang Y..,Wang J..,Lu L..,Li Y..,Wei Y..,...&Ru S..(2020).Genotoxic biomarkers and histological changes in marine medaka (Oryzias melastigma) exposed to 17α-ethynylestradiol and 17β-trenbolone.Marine Pollution Bulletin,150.
MLA	Zhang Y.,et al."Genotoxic biomarkers and histological changes in marine medaka (Oryzias melastigma) exposed to 17α-ethynylestradiol and 17β-trenbolone".Marine Pollution Bulletin 150(2020).