气候变化领域集成服务门户(CCPortal): Transcriptomic analysis of the mode of action of the candidate anti-fouling compound di(1H-indol-3-yl)methane (DIM) on a marine biofouling species; the bryozoan Bugula neritina

CCPortal

DOI	10.1016/j.marpolbul.2020.110904
	Transcriptomic analysis of the mode of action of the candidate anti-fouling compound di(1H-indol-3-yl)methane (DIM) on a marine biofouling species; the bryozoan Bugula neritina
	Xu Y.; Zhang L.; Wang K.-L.; Zhang Y.; Wong Y.H.
发表日期	2020
ISSN	0025326X
卷号	152
英文摘要	Di(1H-indol-3-yl)methane (DIM) was previously suggested to be an environmentally friendly antifouling compound, but it was also reported that the compound was highly stable in natural seawater. The present study reported that 3 h DIM treatments at 4 μg mL−1 or higher concentration and 12 h DIM treatments at 2 μg mL−1 or higher concentration induced significant larval mortality and metamorphic abnormality in the bryozoan Bugula neritina. The bioassay results correlated with the dose-dependent up-regulation of HSP family proteins, pro-apoptotic proteins, ubiquitination protein, and the dose-dependent down-regulation of anti-apoptotic genes and developmental genes. Unexpectedly, genes involved in fatty acid biosynthesis and protein synthesis were up-regulated in response to DIM treatment, but, in general, the effects of DIM on B. neritina larvae were comparable to that reported in human cancer cell lines. DIM also induced changes in steroid hormone biosynthesis genes in B. neritina larvae, leading to the concern that DIM might have long-term effects on marine lives. Overall, the present study suggested that application of DIM to the bryozoan larvae would trigger a major transcriptomic response, which might be linked to the observed larval mortality and abnormality. We suggest that application of DIM as an antifouling ingredient should be proceeded with great cautions. © 2020
英文关键词	Antifouling; Bugula neritina; Mode of action; Settlement; Transcriptome
语种	英语
scopus关键词	Biochemistry; Cell culture; Fatty acids; Genes; Methane; Proteins; Steroid hormones; Antifouling; Bugula neritina; Mode of action; Settlement; Transcriptomes; Biosynthesis; di(1h indol 3 yl)methane; heat shock protein; methane; unclassified drug; methane; transcriptome; antifouling; bioassay; biofouling; bryozoan; concentration (composition); fatty acid; hormone; larva; methane; RNA; sessile species; steroid; adult; antiproliferative activity; Article; bioassay; biofouling; Bugula neritina; cancer cell line; concentration (parameter); controlled study; correlational study; down regulation; fatty acid synthesis; gene; gene expression; gene function; human; human cell; larva; mortality; nonhuman; protein synthesis; transcriptomics; treatment response; ubiquitination; upregulation; animal; Bryozoa; Bryozoa; Bugula neritina; Animals; Biofouling; Bryozoa; Larva; Methane; Transcriptome
来源期刊	Marine Pollution Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/149165
作者单位	Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, China; Institute of Materia Medica, School of Pharmacy and Chemistry, Dali University, Dali, 671000, China; Institute for Advanced Study, Shenzhen University, Shenzhen, 518060, China
推荐引用方式 GB/T 7714	Xu Y.,Zhang L.,Wang K.-L.,et al. Transcriptomic analysis of the mode of action of the candidate anti-fouling compound di(1H-indol-3-yl)methane (DIM) on a marine biofouling species; the bryozoan Bugula neritina[J],2020,152.
APA	Xu Y.,Zhang L.,Wang K.-L.,Zhang Y.,&Wong Y.H..(2020).Transcriptomic analysis of the mode of action of the candidate anti-fouling compound di(1H-indol-3-yl)methane (DIM) on a marine biofouling species; the bryozoan Bugula neritina.Marine Pollution Bulletin,152.
MLA	Xu Y.,et al."Transcriptomic analysis of the mode of action of the candidate anti-fouling compound di(1H-indol-3-yl)methane (DIM) on a marine biofouling species; the bryozoan Bugula neritina".Marine Pollution Bulletin 152(2020).