气候变化领域集成服务门户(CCPortal): Community dynamics and activity of nirS-harboring denitrifiers in sediments of the Indus River Estuary

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DOI	10.1016/j.marpolbul.2020.110971
	Community dynamics and activity of nirS-harboring denitrifiers in sediments of the Indus River Estuary
	Fozia; Zheng Y.; Hou L.; Zhang Z.; Gao D.; Yin G.; Han P.; Dong H.; Liang X.; Yang Y.; Liu M.
发表日期	2020
ISSN	0025326X
卷号	153
英文摘要	Denitrification is an important pathway for reactive nitrogen removal from aquatic ecosystems. In this study, the biodiversity, abundance, and activity of cytochrome cd1-type nitrate reductase gene (nirS)-harboring denitrifiers in the sediments of the Indus River Estuary were examined by molecular and isotope-tracing techniques. Results showed that the nirS-harboring denitrifier communities showed significant geographical variations along the estuarine salinity gradient. Real-time quantitative PCR showed that the abundance of nirS-harboring denitrifiers ranged from 5.3 × 106 to 2.5 × 108 copies g−1, without significant spatiotemporal variation. The potential rates of denitrification varied from 0.01 to 6.27 μmol N kg−1 h−1 and correlated significantly to TOC and Fe(II) (P < 0.05). On the basis of 15N isotope-tracing experiments, the denitrification process contributed 18.4–99.4% to the total nitrogen loss in the sediments of the Indus River Estuary. This study provides novel insights into the microbial mechanism of nitrogen removal process in estuarine ecosystems. © 2020 Elsevier Ltd
英文关键词	Denitrification; nirS gene; Nitrogen; The Indus River Estuary
语种	英语
scopus关键词	Aquatic ecosystems; Biodiversity; Estuaries; Genes; Iron compounds; Isotopes; Nitrogen; Nitrogen removal; Polymerase chain reaction; Rivers; Sediments; Community dynamics; Denitrification process; Geographical variations; Indus River; Nitrogen removal process; Real-time quantitative PCR; Salinity gradients; Spatio-temporal variation; Denitrification; cytochrome; ferrous ion; nitrate reductase; nitrogen 15; nitrate reductase; nitrogen; community response; denitrification; estuarine environment; estuarine sediment; gene; geographical variation; isotopic analysis; microbial activity; microbial community; nitrate; nitrifying bacterium; salinity; sediment pollution; Article; bacterial gene; bacterial strain; biodiversity; community dynamics; controlled study; denitrification; environmental factor; enzyme activity; estuary; geographical variation (species); isotope tracing; microbial activity; molecular biology; nirS gene; nonhuman; population abundance; real time polymerase chain reaction; salinity; sediment; species distribution; waste water management; ecosystem; environmental monitoring; genetics; microbiology; river; sediment; Indus River; Denitrification; Ecosystem; Environmental Monitoring; Estuaries; Geologic Sediments; Nitrate Reductase; Nitrogen; Rivers
来源期刊	Marine Pollution Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/149133
作者单位	State Key Laboratory of Estuarine and Coastal Research, East China Normal University, 500 Dongchuan Road, Minhang District, Shanghai, 200241, China; School of Geographic Sciences, East China Normal University, 500 Dongchuan Road, Minhang District, Shanghai, 200241, China; Key Laboratory of Geographic Information Science (Ministry of Education), East China Normal University, 500 Dongchuan Road, Minhang District, Shanghai, 200241, China
推荐引用方式 GB/T 7714	Fozia,Zheng Y.,Hou L.,et al. Community dynamics and activity of nirS-harboring denitrifiers in sediments of the Indus River Estuary[J],2020,153.
APA	Fozia.,Zheng Y..,Hou L..,Zhang Z..,Gao D..,...&Liu M..(2020).Community dynamics and activity of nirS-harboring denitrifiers in sediments of the Indus River Estuary.Marine Pollution Bulletin,153.
MLA	Fozia,et al."Community dynamics and activity of nirS-harboring denitrifiers in sediments of the Indus River Estuary".Marine Pollution Bulletin 153(2020).