气候变化领域集成服务门户(CCPortal): Generation of albino via SLC45a2 gene targeting by CRISPR/Cas9 in the marine medaka Oryzias melastigma

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DOI	10.1016/j.marpolbul.2020.111038
	Generation of albino via SLC45a2 gene targeting by CRISPR/Cas9 in the marine medaka Oryzias melastigma
	Jeong C.-B.; Kang H.-M.; Hong S.-A.; Byeon E.; Lee J.-S.; Lee Y.H.; Choi I.-Y.; Bae S.; Lee J.-S.
发表日期	2020
ISSN	0025326X
卷号	154
英文摘要	To produce albinism in the marine medaka Oryzias melastigma, we disrupted the solute carrier family 45 (SLC45a2) gene by clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 with a single guide RNA (sgRNA). Selected sgRNAs were able to target a SLC45a2 gene as confirmed by genotyping and heteroduplex mobility assay (HMA). Of the survived embryos after injection, 54.2% and 60.0% embryos exhibited albinism phenotype by sgRNA1 and sgRNA2, respectively. Deep sequencing at the on-target sites showed different insertion and deletion (indel) mutation profiles near the DNA cleavage sites, indicating high efficacy of producing SLC45a2 knock-out mutants by this method. Moreover, HMA at the potential off-target sites revealed that off-target activity would be induced at a low rate, or not induced at all. This albino marine medaka will be a good model for marine molecular ecotoxicology in establishment of diverse in vivo endpoints, and the application of this efficient gene targeting method in the marine medaka would be useful tool for mechanistic approaches. © 2020 Elsevier Ltd
英文关键词	Albinism; CRISPR/Cas9; Marine medaka; SLC45a2
语种	英语
scopus关键词	Gene encoding; Albinism; CRISPR/Cas9; Knockout mutant; Marine medaka; Oryzias melastigma; SLC45a2; Solute carriers; Target activity; Genetic engineering; albinism; DNA; fish; gene expression; mutation; phenotype; RNA; animal cell; Article; clustered regularly interspaced short palindromic repeat; controlled study; DNA cleavage; ecotoxicity; embryo; gene; gene deletion; gene editing; gene insertion sequence; gene sequence; gene targeting; genetic variability; genotype phenotype correlation; in vivo study; nonhuman; Oryzias melastigma; SLC45a2 gene; albinism; animal; clustered regularly interspaced short palindromic repeat; CRISPR Cas system; gene targeting; Oryzias; Oryzias melastigma; Oryziinae; Albinism; Animals; Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR-Cas Systems; Gene Targeting; Oryzias
来源期刊	Marine Pollution Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/149072
作者单位	Department of Biological Science, College of Science, Sungkyunkwan University, Suwon, 16419, South Korea; Department of Marine Science, College of Nature Science, Incheon National University, Incheon, 22012, South Korea; Department of Chemistry, College of Nature Sciences, Hanyang University, Seoul, 04763, South Korea; Department of Agriculture and Life Industry, Kangwon National University, Chuncheon, 24341, South Korea
推荐引用方式 GB/T 7714	Jeong C.-B.,Kang H.-M.,Hong S.-A.,et al. Generation of albino via SLC45a2 gene targeting by CRISPR/Cas9 in the marine medaka Oryzias melastigma[J],2020,154.
APA	Jeong C.-B..,Kang H.-M..,Hong S.-A..,Byeon E..,Lee J.-S..,...&Lee J.-S..(2020).Generation of albino via SLC45a2 gene targeting by CRISPR/Cas9 in the marine medaka Oryzias melastigma.Marine Pollution Bulletin,154.
MLA	Jeong C.-B.,et al."Generation of albino via SLC45a2 gene targeting by CRISPR/Cas9 in the marine medaka Oryzias melastigma".Marine Pollution Bulletin 154(2020).