气候变化领域集成服务门户(CCPortal): Cloning and molecular characterization of estrogen-related receptor (ERR) and vitellogenin genes in the brackish water flea Diaphanosoma celebensis exposed to bisphenol A and its structural analogues

CCPortal

DOI	10.1016/j.marpolbul.2020.111063
	Cloning and molecular characterization of estrogen-related receptor (ERR) and vitellogenin genes in the brackish water flea Diaphanosoma celebensis exposed to bisphenol A and its structural analogues
	In S.; Cho H.; Lee K.-W.; Won E.-J.; Lee Y.-M.
发表日期	2020
ISSN	0025326X
卷号	154
英文摘要	Although it has previously been shown that bisphenol (BP) analogues may interfere with the normal hormonal regulation by acting as endocrine disrupting chemicals (EDCs), little information is available on effects of BP analogues in invertebrates, particularly on cladocerans. In the present study, we identified estrogen-related receptors (EER), vitellogenin (VTG), and VTG receptor (VtgR) from the brackish water flea Diaphanosoma celebensis, and examined the effects of BPA and the substitutes, BPF and BPS, in different sublethal concentrations. Gene expression varied with time well matched with brooding, suggesting that DcEER, DcVTG, and DcVtgR play a role in reproduction in D. celebensis. qRT-PCR analysis showed that BPA and its substitutes differently modulated mRNA expressions of DcEER, DcVTG, and DcVtgR, indicating that these compounds adversely affect the normal reproduction-related pathway. This study facilitates better understanding of the molecular mode of action of BP analogues on the reproductive system of D. celebensis. © 2020
英文关键词	Bisphenol analogues; Brackish water flea; Diaphanosoma celebensis; Estrogen-related receptor; Vitellogenin
语种	英语
scopus关键词	Cell proliferation; Clone cells; Gene expression; Phenols; Bisphenols; Brackish water; Diaphanosoma celebensis; Estrogen-related receptor; Vitellogenin; Endocrine disrupters; 4,4' isopropylidenediphenol; bisphenol F; bisphenol S; complementary DNA; membrane receptor; messenger RNA; phenol derivative; unclassified drug; vitellogenin; vitellogenin receptor; 4,4' isopropylidenediphenol; benzhydryl derivative; endocrine disruptor; estrogen; phenol derivative; salt water; vitellogenin; analog model; brackish water; chemical compound; clone; concentration (composition); crustacean; estrogenic compound; gene expression; genetic engineering; microorganism; protein; RNA; animal experiment; Article; brooding (eggs); Cladocera; concentration (parameter); controlled study; Diaphanosoma celebensis; estrogen related receptor gene; gene; gene expression; molecular cloning; nonhuman; phylogeny; polymerase chain reaction; reproductive toxicity; reverse transcription polymerase chain reaction; RNA extraction; sequence analysis; vitellogenin gene; VTG receptor gene; animal; flea; molecular cloning; water pollutant; Cladocera; Diaphanosoma celebensis; Invertebrata; Animals; Benzhydryl Compounds; Cloning, Molecular; Endocrine Disruptors; Estrogens; Phenols; Saline Waters; Siphonaptera; Vitellogenins; Water Pollutants, Chemical
来源期刊	Marine Pollution Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/149053
作者单位	Department of Biotechnology, College of Convergence Engineering, Sangmyung University, Seoul, 03016, South Korea; Korea Institute of Ocean Science and Technology, 385, Haeyang-ro, Youngdo, Busan, 49111, South Korea; Department of Marine Science & Convergence Engineering, Hanyang University, Ansan, 15588, South Korea
推荐引用方式 GB/T 7714	In S.,Cho H.,Lee K.-W.,et al. Cloning and molecular characterization of estrogen-related receptor (ERR) and vitellogenin genes in the brackish water flea Diaphanosoma celebensis exposed to bisphenol A and its structural analogues[J],2020,154.
APA	In S.,Cho H.,Lee K.-W.,Won E.-J.,&Lee Y.-M..(2020).Cloning and molecular characterization of estrogen-related receptor (ERR) and vitellogenin genes in the brackish water flea Diaphanosoma celebensis exposed to bisphenol A and its structural analogues.Marine Pollution Bulletin,154.
MLA	In S.,et al."Cloning and molecular characterization of estrogen-related receptor (ERR) and vitellogenin genes in the brackish water flea Diaphanosoma celebensis exposed to bisphenol A and its structural analogues".Marine Pollution Bulletin 154(2020).