气候变化领域集成服务门户(CCPortal): Human norovirus detection in bivalve shellfish in Brazil and evaluation of viral infectivity using PMA treatment

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DOI	10.1016/j.marpolbul.2020.111315
	Human norovirus detection in bivalve shellfish in Brazil and evaluation of viral infectivity using PMA treatment
	Sarmento S.K.; Guerra C.R.; Malta F.C.; Coutinho R.; Miagostovich M.P.; Fumian T.M.
发表日期	2020
ISSN	0025326X
卷号	157
英文摘要	Noroviruses are the most common cause of gastroenteritis outbreaks in humans and bivalve shellfish consumption is a recognized route of infection. Our aim was to detect and characterize norovirus in bivalves from a coastal city of Brazil. Nucleic acid was extracted from the bivalve's digestive tissue concentrates using magnetic beads. From March 2018 to June 2019, 77 samples were screened using quantitative RT-PCR. Noroviruses were detected in 41.5%, with the GII being the most prevalent (37.7%). The highest viral load was 3.5 × 106 and 2.5 × 105 GC/g in oysters and mussels, respectively. PMA-treatment demonstrated that a large fraction of the detected norovirus corresponded to non-infectious particles. Genetic characterization showed the circulation of the GII.2[P16] and GII.4[P4] genotypes. Norovirus detection in bivalves reflects the anthropogenic impact on marine environment and serves as an early warning for the food-borne disease outbreaks resulting from the consumption of contaminated molluscs. © 2020 Elsevier Ltd
英文关键词	Marine reserve; Mussels; Norovirus; Oysters; PMA treatment; Rio de Janeiro
语种	英语
scopus关键词	Polymerase chain reaction; Anthropogenic impacts; Food borne disease; Genetic characterization; Infectious particles; Marine environment; Norovirus detections; Quantitative RT-PCR; Shellfish consumption; Molluscs; propidium monoazide; reagent; unclassified drug; virus RNA; concentration (composition); consumption behavior; detection method; disease transmission; genotype; infectious disease; polymerase chain reaction; shellfish culture; viral disease; animal tissue; Article; Brazil; contaminated shellfish; controlled study; environmental impact; genetic variability; genotype; nonhuman; Norovirus; oyster; Perna perna; phylogeny; pseudochama cristella; quantitative analysis; seashore; virus detection; virus infectivity; virus load; virus particle; animal; bivalve; genetics; human; Norovirus; shellfish; Bivalvia; Mollusca; Norovirus; Ostreidae; Animals; Bivalvia; Brazil; Genotype; Humans; Norovirus; RNA, Viral; Shellfish
来源期刊	Marine Pollution Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/148846
作者单位	Laboratório de Virologia Comparada e Ambiental, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ CEP 21045-900, Brazil; Laboratório de Genética Marinha, Departamento de Biotecnologia Marinha, Departamento de Biotecnologia Marinha, Instituto de Estudos do Mar Almirante Paulo Moreira (IEAPM), Arraial do Cabo, RJ CEP 28930-000, Brazil
推荐引用方式 GB/T 7714	Sarmento S.K.,Guerra C.R.,Malta F.C.,et al. Human norovirus detection in bivalve shellfish in Brazil and evaluation of viral infectivity using PMA treatment[J],2020,157.
APA	Sarmento S.K.,Guerra C.R.,Malta F.C.,Coutinho R.,Miagostovich M.P.,&Fumian T.M..(2020).Human norovirus detection in bivalve shellfish in Brazil and evaluation of viral infectivity using PMA treatment.Marine Pollution Bulletin,157.
MLA	Sarmento S.K.,et al."Human norovirus detection in bivalve shellfish in Brazil and evaluation of viral infectivity using PMA treatment".Marine Pollution Bulletin 157(2020).