气候变化领域集成服务门户(CCPortal): Effects of single and combined exposures of gold (nano versus ionic form) and gemfibrozil in a liver organ culture of Sparus aurata

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DOI	10.1016/j.marpolbul.2020.111665
	Effects of single and combined exposures of gold (nano versus ionic form) and gemfibrozil in a liver organ culture of Sparus aurata
	Barreto A.; Carvalho A.; Silva D.; Pinto E.; Almeida A.; Paíga P.; Correira-Sá L.; Delerue-Matos C.; Trindade T.; Soares A.M.V.M.; Hylland K.; Loureiro S.; Oliveira M.
发表日期	2020
ISSN	0025326X
卷号	160
英文摘要	In vitro methods have gained rising importance in ecotoxicology due to ethical concerns. The aim of this study was to assess the single and combined in vitro effects of gold, as nanoparticle (AuNPs) and ionic (Au+) form, and the pharmaceutical gemfibrozil (GEM). Sparus aurata liver organ culture was exposed to gold (4 to 7200 μg·L−1), GEM (1.5 to 15,000 μg·L−1) and combination 80 μg·L−1 gold +150 μg·L−1 GEM for 24 h. Endpoints related with antioxidant status, peroxidative/genetic damage were assessed. AuNPs caused more effects than Au+, increasing catalase and glutathione reductase activities and damaging DNA and cellular membranes. Effects were dependent on AuNPs size, coating and concentration. GEM damaged DNA at an environmentally relevant concentration, 1.5 μg·L−1. Overall, the effects of the combined exposures were higher than the predicted, based on single exposures. This study showed that liver culture can be a useful model to study contaminants effects. © 2020
英文关键词	DNA integrity; Fish liver culture; Gilthead seabream; Ionic gold; Nanotoxicology; Oxidative stress
语种	英语
scopus关键词	Contamination; Marine pollution; Antioxidant status; Cellular membranes; Eco-toxicology; Ethical concerns; Glutathione reductase; Liver culture; Organ culture; Single exposure; Damage detection; catalase; gemfibrozil; glutathione reductase; gold nanoparticle; bioaccumulation; biological uptake; concentration (composition); drug; genotoxicity; gold; ionic composition; nanoparticle; oxidative stress; perciform; pollution exposure; toxicology; animal tissue; Article; cell membrane; concentration (parameter); controlled study; DNA damage; enzyme activity; high performance liquid chromatography; limit of quantitation; liver; melting point; nonhuman; organ culture; particle size; prediction; room temperature; Sparus aurata; toxicity testing; transmission electron microscopy; Sparus aurata
来源期刊	Marine Pollution Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/148545
作者单位	Departamento de Biologia & CESAM, Universidade de Aveiro, Aveiro, 3810-193, Portugal; Departamento de Saúde Ambiental, Escola Superior de Saúde, P. Porto. CISA/Centro de Investigação em saúde e Ambiente, Rua Dr. António Bernardino de Almeida, 400, Porto, 4200-072, Portugal; LAQV/REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua Jorge Viterbo Ferreira, 228, Porto, 4050-313, Portugal; REQUIMTE/LAQV, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida, 431, Porto, 4200-072, Portugal; Departamento de Química & CICECO - Aveiro Instituto de Materiais, Universidade de Aveiro, Aveiro, 3810-193, Portugal; Department of Biosciences, University of Oslo, PO Box 1066, Oslo, N-0316, Norway
推荐引用方式 GB/T 7714	Barreto A.,Carvalho A.,Silva D.,et al. Effects of single and combined exposures of gold (nano versus ionic form) and gemfibrozil in a liver organ culture of Sparus aurata[J],2020,160.
APA	Barreto A..,Carvalho A..,Silva D..,Pinto E..,Almeida A..,...&Oliveira M..(2020).Effects of single and combined exposures of gold (nano versus ionic form) and gemfibrozil in a liver organ culture of Sparus aurata.Marine Pollution Bulletin,160.
MLA	Barreto A.,et al."Effects of single and combined exposures of gold (nano versus ionic form) and gemfibrozil in a liver organ culture of Sparus aurata".Marine Pollution Bulletin 160(2020).