气候变化领域集成服务门户(CCPortal): Bacterial community composition and diversity in the ballast water of container ships arriving at Yangshan Port; Shanghai; China

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DOI	10.1016/j.marpolbul.2020.111640
	Bacterial community composition and diversity in the ballast water of container ships arriving at Yangshan Port; Shanghai; China
	Wang Q.; Cheng F.; Xue J.; Xiao N.; Wu H.
发表日期	2020
ISSN	0025326X
卷号	160
英文摘要	Ballast water is a major vector of invasion by protozoans and metazoans. Bacterial invasion is less-well understood. We surveyed the bacterial diversity of ballast water from 26 container ships arriving at the Yangshan Deepwater Port, Shanghai, China during 2015–2016. We characterized the ballast microbiome using high-throughput sequencing (HTS) based on V4-V5 region of 16S rRNA genes. We simultaneously monitored physicochemical parameters of the ballast water, including temperature, pH, dissolved oxygen (DO), salinity, turbidity, total suspended solid (TSS), particulate organic carbon (POC), NO2, NH4, PO4. Proteobacteria was the dominant phylum, comprising more than 50% of the OTUs of almost all vessels, followed by Bacteroidetes (12.08%), Actinobacteria (4.86%) Planctomycetes (3.24%) and Cyanobacteria (1.95%). The relative abundance of Cyanobacteria differed among vessels. It was negatively correlated with temperature, NO3, pH, TSS, PO4, and turbidity and positively correlated with NH4, POC. The genus Synechococcus was the most common Cyanobacteria in our results. Escherichia coli were relatively rare; they are indicator-species of D-2 standards published by the IMO. The relative abundance of the genus Vibrio ranged from 0.003% to 24.88% among different vessels. Our results showed that HTS was able to profile the bacterial communities in ballast-waters, even when the approach was restricted by technical and other obstacles. © 2020 Elsevier Ltd
英文关键词	16S rRNA; Ballast water; High-throughput sequencing; Microbial community; Yangshan Port
语种	英语
scopus关键词	Biochemical oxygen demand; Containers; Dissolved oxygen; Escherichia coli; Forestry; Organic carbon; Oxygenators; RNA; Ships; Turbidity; Water; Bacterial community; Bacterial community composition; Bacterial diversity; Bacterial invasion; High-throughput sequencing; Particulate organic carbon; Physicochemical parameters; Total suspended solids; Ballast (railroad track); bacterium; ballast water; community composition; container ship; microbial community; physicochemical property; port; RNA; species diversity; China; Shanghai; Actinobacteria; Bacteria (microorganisms); Bacteroidetes; Cyanobacteria; Escherichia coli; Metazoa; Otus; Planctomycetes; Proteobacteria; Protozoa; Synechococcus; Vibrio
来源期刊	Marine Pollution Bulletin
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/148493
作者单位	College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai, 201306, China; Centre for Research on the Ecological Security of Ports and Shipping, Shanghai Ocean University, Shanghai, 201306, China
推荐引用方式 GB/T 7714	Wang Q.,Cheng F.,Xue J.,et al. Bacterial community composition and diversity in the ballast water of container ships arriving at Yangshan Port; Shanghai; China[J],2020,160.
APA	Wang Q.,Cheng F.,Xue J.,Xiao N.,&Wu H..(2020).Bacterial community composition and diversity in the ballast water of container ships arriving at Yangshan Port; Shanghai; China.Marine Pollution Bulletin,160.
MLA	Wang Q.,et al."Bacterial community composition and diversity in the ballast water of container ships arriving at Yangshan Port; Shanghai; China".Marine Pollution Bulletin 160(2020).