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DOI	10.5194/acp-20-5197-2020
	Characterization and comparison of PM2.5 oxidative potential assessed by two acellular assays
	Gao D.; J. Godri Pollitt K.; A. Mulholland J.; G. Russell A.; J. Weber R.
发表日期	2020
ISSN	1680-7316
起始页码	5197
结束页码	5210
卷号	20 期号:9
英文摘要	The capability of ambient particles to generate in vivo reactive oxygen species (ROS), called oxidative potential (OP), is a potential metric for evaluating the health effects of particulate matter (PM) and is supported by several recent epidemiological investigations. Studies using various types of OP assays differ in their sensitivities to varying PM chemical components. In this study, we systematically compared two health-relevant acellular OP assays that track the depletion of antioxidants or reductant surrogates: (i) the synthetic respiratory-tract lining fluid (RTLF) assay that tracks the depletion of ascorbic acid (AA) and glutathione (GSH) and (ii) the dithiothreitol (DTT) assay that tracks the depletion of DTT. Yearlong daily samples were collected at an urban site in Atlanta, GA (Jefferson Street), during 2017, and both DTT and RTLF assays were performed to measure the OP of water-soluble PM2.5 components. PM2.5 mass and major chemical components, including metals, ions, and organic and elemental carbon were also analyzed. Correlation analysis found that OP as measured by the DTT and AA depletion (OPDTT and OPAA, respectively) were correlated with both organics and some water-soluble metal species, whereas that from the GSH depletion (OPGSH) was exclusively sensitive to water-soluble Cu. These OP assays were moderately correlated with each other due to the common contribution from metal ions. OPDTT and OPAA were moderately correlated with PM2.5 mass with Pearson's rCombining double low line0.55 and 0.56, respectively, whereas OPGSH exhibited a lower correlation (rCombining double low line0.24). There was little seasonal variation in the OP levels for all assays due to the weak seasonality of OP-associated species. Multivariate linear regression models were developed to predict OP measures from the particle composition data. Variability in OPDTT and OPAA were not only attributed to the concentrations of metal ions (mainly Fe and Cu) and organic compounds but also to antagonistic metal-organic and metal-metal interactions. OPGSH was sensitive to the change in water-soluble Cu and brown carbon (BrC), a proxy for ambient humic-like substances. © 2020 Copernicus GmbH. All rights reserved.
语种	英语
scopus关键词	bioassay; multivariate analysis; particulate matter; reactive oxygen species; seasonal variation; seasonality
来源期刊	Atmospheric Chemistry and Physics
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/141373
作者单位	School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, GA 30332, United States; Department of Environmental Health Sciences, School of Public Health, Yale University, New Haven, CT 06510, United States; School of Earth and Atmospheric Sciences, Georgia Institute of Technology, Atlanta, GA 30332, United States; Department of Environmental Health Sciences, School of Public Health, Yale University, New Haven, CT 06510, United States
推荐引用方式 GB/T 7714	Gao D.,J. Godri Pollitt K.,A. Mulholland J.,et al. Characterization and comparison of PM2.5 oxidative potential assessed by two acellular assays[J],2020,20(9).
APA	Gao D.,J. Godri Pollitt K.,A. Mulholland J.,G. Russell A.,&J. Weber R..(2020).Characterization and comparison of PM2.5 oxidative potential assessed by two acellular assays.Atmospheric Chemistry and Physics,20(9).
MLA	Gao D.,et al."Characterization and comparison of PM2.5 oxidative potential assessed by two acellular assays".Atmospheric Chemistry and Physics 20.9(2020).