气候变化领域集成服务门户(CCPortal): Single-cell transcriptional diversity is a hallmark of developmental potential

CCPortal

DOI	10.1126/science.aax0249
	Single-cell transcriptional diversity is a hallmark of developmental potential
	Gulati G.S.; Sikandar S.S.; Wesche D.J.; Manjunath A.; Bharadwaj A.; Berger M.J.; Ilagan F.; Kuo A.H.; Hsieh R.W.; Cai S.; Zabala M.; Scheeren F.A.; Lobo N.A.; Qian D.; Yu F.B.; Dirbas F.M.; Clarke M.F.; Newman A.M.
发表日期	2020
ISSN	0036-8075
起始页码	405
结束页码	411
卷号	367 期号:6476
英文摘要	Single-cell RNA sequencing (scRNA-seq) is a powerful approach for reconstructing cellular differentiation trajectories. However, inferring both the state and direction of differentiation is challenging. Here, we demonstrate a simple, yet robust, determinant of developmental potential—the number of expressed genes per cell—and leverage this measure of transcriptional diversity to develop a computational framework (CytoTRACE) for predicting differentiation states from scRNA-seq data. When applied to diverse tissue types and organisms, CytoTRACE outperformed previous methods and nearly 19,000 annotated gene sets for resolving 52 experimentally determined developmental trajectories. Additionally, it facilitated the identification of quiescent stem cells and revealed genes that contribute to breast tumorigenesis. This study thus establishes a key RNA-based feature of developmental potential and a platform for delineation of cellular hierarchies. Copyright © 2020 The Authors,
关键词	cell cell component developmental biology experimental study genetic analysis tumor Article breast cancer breast carcinogenesis carcinogenesis cell differentiation computer model embryonic stem cell gene expression genetic code genetic variability human in vivo study nonhuman phenotype priority journal scoring system single cell RNA seq upregulation
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133828
推荐引用方式 GB/T 7714	Gulati G.S.,Sikandar S.S.,Wesche D.J.,et al. Single-cell transcriptional diversity is a hallmark of developmental potential[J]. Science,2020,367(6476).
APA	Gulati G.S..,Sikandar S.S..,Wesche D.J..,Manjunath A..,Bharadwaj A..,...&Newman A.M..(2020).Single-cell transcriptional diversity is a hallmark of developmental potential.,367(6476).
MLA	Gulati G.S.,et al."Single-cell transcriptional diversity is a hallmark of developmental potential".367.6476(2020).