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| DOI | 10.1126/science.aay1645 |
| Chromatin accessibility dynamics in a model of human forebrain development | |
| Trevino A.E.; Sinnott-Armstrong N.; Andersen J.; Yoon S.-J.; Huber N.; Pritchard J.K.; Chang H.Y.; Greenleaf W.J.; Pașca S.P. | |
| 发表日期 | 2020 |
| ISSN | 0036-8075 |
| 卷号 | 367期号:6476 |
| 英文摘要 | Forebrain development is characterized by highly synchronized cellular processes, which, if perturbed, can cause disease. To chart the regulatory activity underlying these events, we generated a map of accessible chromatin in human three-dimensional forebrain organoids. To capture corticogenesis, we sampled glial and neuronal lineages from dorsal or ventral forebrain organoids over 20 months in vitro. Active chromatin regions identified in human primary brain tissue were observed in organoids at different developmental stages. We used this resource to map genetic risk for disease and to explore evolutionary conservation. Moreover, we integrated chromatin accessibility with transcriptomics to identify putative enhancer-gene linkages and transcription factors that regulate human corticogenesis. Overall, this platform brings insights into gene-regulatory dynamics at previously inaccessible stages of human forebrain development, including signatures of neuropsychiatric disorders. © 2020 American Association for the Advancement of Science. All rights reserved. |
| 关键词 | braincell componentdevelopmental biologydevelopmental stagediseasegenetic analysisnervous system disorderArticlebrain cortexbrain developmentbrain nerve cellbrain tissuecell differentiationcell migrationchromatincontrolled studyenvironmental factorfluorescence activated cell sortingforebrainGABAergic systemglia cellheredityhigh throughput sequencinghumanhuman cellhuman tissuepluripotent stem cellpriority journalRNA isolationRNA sequenceRNA sequencing |
| 语种 | 英语 |
| 来源机构 | Science |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/133821 |
| 推荐引用方式 GB/T 7714 | Trevino A.E.,Sinnott-Armstrong N.,Andersen J.,et al. Chromatin accessibility dynamics in a model of human forebrain development[J]. Science,2020,367(6476). |
| APA | Trevino A.E..,Sinnott-Armstrong N..,Andersen J..,Yoon S.-J..,Huber N..,...&Pașca S.P..(2020).Chromatin accessibility dynamics in a model of human forebrain development.,367(6476). |
| MLA | Trevino A.E.,et al."Chromatin accessibility dynamics in a model of human forebrain development".367.6476(2020). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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