气候变化领域集成服务门户(CCPortal): Electrostatic control of photoisomerization pathways in proteins

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DOI	10.1126/science.aax1898
	Electrostatic control of photoisomerization pathways in proteins
	Romei M.G.; Lin C.-Y.; Mathews I.I.; Boxer S.G.
发表日期	2020
ISSN	0036-8075
起始页码	76
结束页码	79
卷号	367 期号:6473
英文摘要	Rotation around a specific bond after photoexcitation is central to vision and emerging opportunities in optogenetics, super-resolution microscopy, and photoactive molecular devices. Competing roles for steric and electrostatic effects that govern bond-specific photoisomerization have been widely discussed, the latter originating from chromophore charge transfer upon excitation. We systematically altered the electrostatic properties of the green fluorescent protein chromophore in a photoswitchable variant, Dronpa2, using amber suppression to introduce electron-donating and electron-withdrawing groups to the phenolate ring. Through analysis of the absorption (color), fluorescence quantum yield, and energy barriers to ground- and excited-state isomerization, we evaluate the contributions of sterics and electrostatics quantitatively and demonstrate how electrostatic effects bias the pathway of chromophore photoisomerization, leading to a generalized framework to guide protein design. © 2020 American Association for the Advancement of Science. All rights reserved.
关键词	absorption control system fluorescence genetics molecular analysis protein vision article fluorescence isomerization quantitative analysis quantum yield static electricity
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133810
推荐引用方式 GB/T 7714	Romei M.G.,Lin C.-Y.,Mathews I.I.,et al. Electrostatic control of photoisomerization pathways in proteins[J]. Science,2020,367(6473).
APA	Romei M.G.,Lin C.-Y.,Mathews I.I.,&Boxer S.G..(2020).Electrostatic control of photoisomerization pathways in proteins.,367(6473).
MLA	Romei M.G.,et al."Electrostatic control of photoisomerization pathways in proteins".367.6473(2020).