气候变化领域集成服务门户(CCPortal): Effect of tolerance on the evolution of antibiotic resistance under drug combinations

CCPortal

DOI	10.1126/science.aay3041
	Effect of tolerance on the evolution of antibiotic resistance under drug combinations
	Liu J.; Gefen O.; Ronin I.; Bar-Meir M.; Balaban N.Q.
发表日期	2020
ISSN	0036-8075
起始页码	200
结束页码	204
卷号	367 期号:6474
英文摘要	Drug combinations are widely used in clinical practice to prevent the evolution of resistance. However, little is known about the effect of tolerance, a different mode of survival, on the efficacy of drug combinations for preventing the evolution of resistance. In this work, we monitored Staphylococcus aureus strains evolving in patients under treatment. We detected the rapid emergence of tolerance mutations, followed by the emergence of resistance, despite the combination treatment. Evolution experiments on the clinical strains in vitro revealed a new way by which tolerance promotes the evolution of resistance under combination treatments. Further experiments under different antibiotic classes reveal the generality of the effect. We conclude that tolerance is an important factor to consider in designing combination treatments that prevent the evolution of resistance. © 2020 American Association for the Advancement of Science. All rights reserved.
关键词	daptomycin rifampicin vancomycin antiinfective agent beta' subunit of RNA polymerase daptomycin DNA directed RNA polymerase rifampicin vancomycin antibiotic resistance bacterium emergence evolutionary biology experimental study laboratory method survival adult antibiotic resistance Article bacterial mutation bacterial strain bacterium isolation clinical article colony forming unit combination drug therapy drug effect drug tolerance human in vitro study minimum inhibitory concentration nonhuman phylogeny priority journal Staphylococcus aureus whole genome sequencing antibiotic resistance combination drug therapy drug effect genetics methicillin resistant Staphylococcus aureus microbial sensitivity test microbiology molecular evolution mutation single nucleotide polymorphism Staphylococcus infection Staphylococcus aureus Anti-Bacterial Agents Daptomycin DNA-Directed RNA Polymerases Drug Resistance, Microbial Drug Therapy, Combination Evolution, Molecular Humans Methicillin-Resistant Staphylococcus aureus Microbial Sensitivity Tests Mutation Polymorphism, Single Nucleotide Rifampin Staphylococcal Infections Vancomycin
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133802
推荐引用方式 GB/T 7714	Liu J.,Gefen O.,Ronin I.,et al. Effect of tolerance on the evolution of antibiotic resistance under drug combinations[J]. Science,2020,367(6474).
APA	Liu J.,Gefen O.,Ronin I.,Bar-Meir M.,&Balaban N.Q..(2020).Effect of tolerance on the evolution of antibiotic resistance under drug combinations.,367(6474).
MLA	Liu J.,et al."Effect of tolerance on the evolution of antibiotic resistance under drug combinations".367.6474(2020).