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| DOI | 10.1126/science.aax9039 |
| Engineered symbionts activate honey bee immunity and limit pathogens | |
| Leonard S.P.; Powell J.E.; Perutka J.; Geng P.; Heckmann L.C.; Horak R.D.; Davies B.W.; Ellington A.D.; Barrick J.E.; Moran N.A. | |
| 发表日期 | 2020 |
| ISSN | 0036-8075 |
| 起始页码 | 573 |
| 结束页码 | 576 |
| 卷号 | 367期号:6477 |
| 英文摘要 | Honey bees are essential pollinators threatened by colony losses linked to the spread of parasites and pathogens. Here, we report a new approach for manipulating bee gene expression and protecting bee health. We engineered a symbiotic bee gut bacterium, Snodgrassella alvi, to induce eukaryotic RNA interference (RNAi) immune responses. We show that engineered S. alvi can stably recolonize bees and produce double-stranded RNA to activate RNAi and repress host gene expression, thereby altering bee physiology, behavior, and growth. We used this approach to improve bee survival after a viral challenge, and we show that engineered S. alvi can kill parasitic Varroa mites by triggering the mite RNAi response. This symbiont-mediated RNAi approach is a tool for studying bee functional genomics and potentially for safeguarding bee health. © 2020 American Association for the Advancement of Science. All rights reserved. |
| 关键词 | double stranded RNAbacteriumengineeringhoneybeehost-pathogen interactionimmune responseimmunitypathogensymbiontArticlebacterial colonizationcontrolled studygene expressiongene repressiongenetic engineeringhoneybeeimmunityintestine floranonhumanpriority journalRNA interferenceSnodgrassella alvisymbiontVarroaanimalbeegenetic engineeringgeneticsimmunologymicrobiologyNeisseriaceaephysiologysymbiosisVarroidaeAcariApis melliferaApoideaBacteria (microorganisms)EukaryotaVarroaAnimalsBeesGastrointestinal MicrobiomeGenetic EngineeringNeisseriaceaeRNA InterferenceSymbiosisVarroidae |
| 语种 | 英语 |
| 来源机构 | Science |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/133755 |
| 推荐引用方式 GB/T 7714 | Leonard S.P.,Powell J.E.,Perutka J.,et al. Engineered symbionts activate honey bee immunity and limit pathogens[J]. Science,2020,367(6477). |
| APA | Leonard S.P..,Powell J.E..,Perutka J..,Geng P..,Heckmann L.C..,...&Moran N.A..(2020).Engineered symbionts activate honey bee immunity and limit pathogens.,367(6477). |
| MLA | Leonard S.P.,et al."Engineered symbionts activate honey bee immunity and limit pathogens".367.6477(2020). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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