气候变化领域集成服务门户(CCPortal): Monosomes actively translate synaptic mRNAs in neuronal processes

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DOI	10.1126/science.aay4991
	Monosomes actively translate synaptic mRNAs in neuronal processes
	Biever A.; Glock C.; Tushev G.; Ciirdaeva E.; Dalmay T.; Langer J.D.; Schuman E.M.
发表日期	2020
ISSN	0036-8075
卷号	367 期号:6477
英文摘要	To accommodate their complex morphology, neurons localize messenger RNAs (mRNAs) and ribosomes near synapses to produce proteins locally. However, a relative paucity of polysomes (considered the active sites of translation) detected in electron micrographs of neuronal processes has suggested a limited capacity for local protein synthesis. In this study, we used polysome profiling together with ribosome footprinting of microdissected rodent synaptic regions to reveal a surprisingly high number of dendritic and/or axonal transcripts preferentially associated with monosomes (single ribosomes). Furthermore, the neuronal monosomes were in the process of active protein synthesis. Most mRNAs showed a similar translational status in the cell bodies and neurites, but some transcripts exhibited differential ribosome occupancy in the compartments. Monosome-preferring transcripts often encoded high-abundance synaptic proteins. Thus, monosome translation contributes to the local neuronal proteome. © 2020 American Association for the Advancement of Science. All rights reserved.
关键词	proteome cell cell component protein proteomics RNA adult animal cell animal tissue Article controlled study dendrite monosome mouse neuropil nonhuman priority journal protein synthesis rat ribosome RNA transcription RNA translation synapse Rodentia
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133749
推荐引用方式 GB/T 7714	Biever A.,Glock C.,Tushev G.,et al. Monosomes actively translate synaptic mRNAs in neuronal processes[J]. Science,2020,367(6477).
APA	Biever A..,Glock C..,Tushev G..,Ciirdaeva E..,Dalmay T..,...&Schuman E.M..(2020).Monosomes actively translate synaptic mRNAs in neuronal processes.,367(6477).
MLA	Biever A.,et al."Monosomes actively translate synaptic mRNAs in neuronal processes".367.6477(2020).