气候变化领域集成服务门户(CCPortal): N6-methyladenosine of chromosome-associated regulatory RNA regulates chromatin state and transcription

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DOI	10.1126/science.aay6018
	N6-methyladenosine of chromosome-associated regulatory RNA regulates chromatin state and transcription
	Liu J.; Dou X.; Chen C.; Chen C.; Liu C.; Michelle Xu M.; Zhao S.; Shen B.; Gao Y.; Han D.; He C.
发表日期	2020
ISSN	0036-8075
起始页码	580
结束页码	586
卷号	367 期号:6477
英文摘要	N6-methyladenosine (m6A) regulates stability and translation of messenger RNA (mRNA) in various biological processes. In this work, we show that knockout of the m6A writer Mettl3 or the nuclear reader Ythdc1 in mouse embryonic stem cells increases chromatin accessibility and activates transcription in an m6A-dependent manner. We found that METTL3 deposits m6A modifications on chromosome-associated regulatory RNAs (carRNAs), including promoter-associated RNAs, enhancer RNAs, and repeat RNAs. YTHDC1 facilitates the decay of a subset of these m6A-modified RNAs, especially elements of the long interspersed element-1 family, through the nuclear exosome targeting-mediated nuclear degradation. Reducing m6A methylation by METTL3 depletion or site-specific m6A demethylation of selected carRNAs elevates the levels of carRNAs and promotes open chromatin state and downstream transcription. Collectively, our results reveal that m6A on carRNAs can globally tune chromatin state and transcription. © 2020 American Association for the Advancement of Science. All rights reserved.
关键词	6 n methyladenosine chromosome associated regulatory RNA enhancer RNA messenger RNA promoter associated RNA repeat RNA RNA unclassified drug cell component chemical compound chromosome methylation RNA animal cell Article biodegradation chemical modification chromatin controlled study demethylation embryo embryonic stem cell exosome methylation METTL3 gene mouse nonhuman nuclear degradation priority journal transcription regulation Ythdc1 gene
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133747
推荐引用方式 GB/T 7714	Liu J.,Dou X.,Chen C.,et al. N6-methyladenosine of chromosome-associated regulatory RNA regulates chromatin state and transcription[J]. Science,2020,367(6477).
APA	Liu J..,Dou X..,Chen C..,Chen C..,Liu C..,...&He C..(2020).N6-methyladenosine of chromosome-associated regulatory RNA regulates chromatin state and transcription.,367(6477).
MLA	Liu J.,et al."N6-methyladenosine of chromosome-associated regulatory RNA regulates chromatin state and transcription".367.6477(2020).