气候变化领域集成服务门户(CCPortal): Structure of an active human histone pre-mRNA 3′-end processing machinery

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DOI	10.1126/science.aaz7758
	Structure of an active human histone pre-mRNA 3′-end processing machinery
	Sun Y.; Zhang Y.; Aik W.S.; Yang X.-C.; Marzluff W.F.; Walz T.; Dominski Z.; Tong L.
发表日期	2020
ISSN	0036-8075
起始页码	700
结束页码	703
卷号	367 期号:6478
英文摘要	The 3′-end processing machinery for metazoan replication-dependent histone precursor messenger RNAs (pre-mRNAs) contains the U7 small nuclear ribonucleoprotein and shares the key cleavage module with the canonical cleavage and polyadenylation machinery. We reconstituted an active human histone pre-mRNA processing machinery using 13 recombinant proteins and two RNAs and determined its structure by cryo–electron microscopy. The overall structure is highly asymmetrical and resembles an amphora with one long handle. We captured the pre-mRNA in the active site of the endonuclease, the 73-kilodalton subunit of the cleavage and polyadenylation specificity factor, poised for cleavage. The endonuclease and the entire cleavage module undergo extensive rearrangements for activation, triggered through the recognition of the duplex between the authentic pre-mRNA and U7 small nuclear RNA (snRNA). Our study also has notable implications for understanding canonical and snRNA 3′-end processing. Copyright © 2020 The Authors,
关键词	beta lactamase cleavage and polyadenylation specificity factor cleavage and polyadenylation specificity factor 100 cleavage and polyadenylation specificity factor 73 endonuclease histone membrane protein messenger RNA precursor recombinant protein small nuclear ribonucleoprotein small nuclear RNA symplekin U7 small nuclear ribonucleoprotein unclassified drug enzyme activity genetic analysis metazoan protein recombination RNA 3' untranslated region amino terminal sequence Article biochemical analysis carboxy terminal sequence conformational transition controlled study cryoelectron microscopy enzyme active site gene rearrangement genetic analysis hydrogen bond molecular recognition mutagenesis polyadenylation priority journal protein conformation protein protein interaction RNA cleavage RNA processing RNA sequencing Amphora Metazoa
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133734
推荐引用方式 GB/T 7714	Sun Y.,Zhang Y.,Aik W.S.,等. Structure of an active human histone pre-mRNA 3′-end processing machinery[J]. Science,2020,367(6478).
APA	Sun Y..,Zhang Y..,Aik W.S..,Yang X.-C..,Marzluff W.F..,...&Tong L..(2020).Structure of an active human histone pre-mRNA 3′-end processing machinery.,367(6478).
MLA	Sun Y.,et al."Structure of an active human histone pre-mRNA 3′-end processing machinery".367.6478(2020).