气候变化领域集成服务门户(CCPortal): Structure of nucleosome-bound human BAF complex

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DOI	10.1126/science.aaz9761
	Structure of nucleosome-bound human BAF complex
	He S.; Wu Z.; Tian Y.; Yu Z.; Yu J.; Wang X.; Li J.; Liu B.; Xu Y.
发表日期	2020
ISSN	0036-8075
起始页码	875
结束页码	881
卷号	367 期号:6480
英文摘要	Mammalian SWI/SNF family chromatin remodelers, BRG1/BRM-associated factor (BAF) and polybromo-associated BAF (PBAF), regulate chromatin structure and transcription, and their mutations are linked to cancers. The 3.7-angstrom-resolution cryo-electron microscopy structure of human BAF bound to the nucleosome reveals that the nucleosome is sandwiched by the base and the adenosine triphosphatase (ATPase) modules, which are bridged by the actin-related protein (ARP) module. The ATPase motor is positioned proximal to nucleosomal DNA and, upon ATP hydrolysis, engages with and pumps DNA along the nucleosome. The C-terminal a helix of SMARCB1, enriched in positively charged residues frequently mutated in cancers, mediates interactions with an acidic patch of the nucleosome. AT-rich interactive domain-containing protein 1A (ARID1A) and the SWI/SNF complex subunit SMARCC serve as a structural core and scaffold in the base module organization, respectively. Our study provides structural insights into subunit organization and nucleosome recognition of human BAF complex. © 2020 American Association for the Advancement of Science. All rights reserved.
关键词	actin related protein adenosine triphosphatase BRG1 BRM associated factor DNA multiprotein complex protein SWI retinoblastoma binding protein 2 SMARCC protein SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily B member 1 transcription factor transcription factor SNF unclassified drug cancer enzyme activity gene expression hydrolysis mutation protein alpha helix Article carboxy terminal sequence cell interaction cryoelectron microscopy disease association human malignant neoplasm molecular recognition mutation nucleosome priority journal protein function protein hydrolysis protein structure structure analysis Mammalia
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133686
推荐引用方式 GB/T 7714	He S.,Wu Z.,Tian Y.,et al. Structure of nucleosome-bound human BAF complex[J]. Science,2020,367(6480).
APA	He S..,Wu Z..,Tian Y..,Yu Z..,Yu J..,...&Xu Y..(2020).Structure of nucleosome-bound human BAF complex.,367(6480).
MLA	He S.,et al."Structure of nucleosome-bound human BAF complex".367.6480(2020).