气候变化领域集成服务门户(CCPortal): Polymerization in the actin ATPase clan regulates hexokinase activity in yeast

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DOI	10.1126/science.aay5359
	Polymerization in the actin ATPase clan regulates hexokinase activity in yeast
	Stoddard P.R.; Lynch E.M.; Farrell D.P.; dosey A.M.; diMaio F.; Williams T.A.; Kollman J.M.; Murray A.W.; Garner E.C.
发表日期	2020
ISSN	0036-8075
起始页码	1039
结束页码	1042
卷号	367 期号:6481
英文摘要	The actin fold is found in cytoskeletal polymers, chaperones, and various metabolic enzymes. Many actin-fold proteins, such as the carbohydrate kinases, do not polymerize. We found that Glk1, a Saccharomyces cerevisiae glucokinase, forms two-stranded filaments with ultrastructure that is distinct from that of cytoskeletal polymers. In cells, Glk1 polymerized upon sugar addition and depolymerized upon sugar withdrawal. Polymerization inhibits enzymatic activity; the Glk1 monomer-polymer equilibrium sets a maximum rate of glucose phosphorylation regardless of Glk1 concentration. A mutation that eliminated Glk1 polymerization alleviated concentration-dependent enzyme inhibition. Yeast containing nonpolymerizing Glk1 were less fit when growing on sugars and more likely to die when refed glucose. Glk1 polymerization arose independently from other actin-related filaments and may allow yeast to rapidly modulate glucokinase activity as nutrient availability changes. © 2020 American Association for the Advancement of Science. All rights reserved.
关键词	actin adenosine triphosphatase Glk1 protein glucokinase glucose hexokinase monomer polymer unclassified drug carbohydrate cell component enzyme enzyme activity mutation nutrient availability polymer polymerization ultrastructure actin filament actin polymerization Article depolymerization enzyme activity enzyme inhibition enzyme phosphorylation nonhuman nutrient availability priority journal protein blood level Saccharomyces cerevisiae Saccharomyces cerevisiae
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133673
推荐引用方式 GB/T 7714	Stoddard P.R.,Lynch E.M.,Farrell D.P.,et al. Polymerization in the actin ATPase clan regulates hexokinase activity in yeast[J]. Science,2020,367(6481).
APA	Stoddard P.R..,Lynch E.M..,Farrell D.P..,dosey A.M..,diMaio F..,...&Garner E.C..(2020).Polymerization in the actin ATPase clan regulates hexokinase activity in yeast.,367(6481).
MLA	Stoddard P.R.,et al."Polymerization in the actin ATPase clan regulates hexokinase activity in yeast".367.6481(2020).