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DOI | 10.1126/science.aay0262 |
Pervasive functional translation of noncanonical human open reading frames | |
Chen J.; Brunner A.-D.; Cogan J.Z.; Nuñez J.K.; Fields A.P.; Adamson B.; Itzhak D.N.; Li J.Y.; Mann M.; Leonetti M.D.; Weissman J.S. | |
发表日期 | 2020 |
ISSN | 10959203 |
起始页码 | 1140 |
结束页码 | 1146 |
卷号 | 367期号:6482 |
英文摘要 | Ribosome profiling has revealed pervasive but largely uncharacterized translation outside of canonical coding sequences (CDSs). In this work, we exploit a systematic CRISPR-based screening strategy to identify hundreds of noncanonical CDSs that are essential for cellular growth and whose disruption elicits specific, robust transcriptomic and phenotypic changes in human cells. Functional characterization of the encoded microproteins reveals distinct cellular localizations, specific protein binding partners, and hundreds of microproteins that are presented by the human leukocyte antigen system. We find multiple microproteins encoded in upstream open reading frames, which form stable complexes with the main, canonical protein encoded on the same messenger RNA, thereby revealing the use of functional bicistronic operons in mammals. Together, our results point to a family of functional human microproteins that play critical and diverse cellular roles. Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. |
关键词 | messenger RNApeptidetranscriptomeCRISPR Cas systemgeneticshumanmetabolismopen reading frameoperonprotein synthesisribosomeCRISPR-Cas SystemsHumansOpen Reading FramesOperonPeptidesProtein BiosynthesisRibosomesRNA, MessengerTranscriptome |
语种 | 英语 |
来源机构 | Science (New York, N.Y.) |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/133663 |
推荐引用方式 GB/T 7714 | Chen J.,Brunner A.-D.,Cogan J.Z.,et al. Pervasive functional translation of noncanonical human open reading frames[J]. Science (New York, N.Y.),2020,367(6482). |
APA | Chen J..,Brunner A.-D..,Cogan J.Z..,Nuñez J.K..,Fields A.P..,...&Weissman J.S..(2020).Pervasive functional translation of noncanonical human open reading frames.,367(6482). |
MLA | Chen J.,et al."Pervasive functional translation of noncanonical human open reading frames".367.6482(2020). |
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