气候变化领域集成服务门户(CCPortal): PE/PPE proteins mediate nutrient transport across the outer membrane of Mycobacterium tuberculosis

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DOI	10.1126/science.aax3072
	PE/PPE proteins mediate nutrient transport across the outer membrane of Mycobacterium tuberculosis
	Wang Q.; Boshoff H.I.M.; Harrison J.R.; Ray P.C.; Green S.R.; Wyatt P.G.; Barry Iii C.E.
发表日期	2020
ISSN	0036-8075
起始页码	1147
结束页码	1151
卷号	367 期号:6482
英文摘要	Mycobacterium tuberculosis has an unusual outer membrane that lacks canonical porin proteins for the transport of small solutes to the periplasm. We discovered that 3,3-bis-di(methylsulfonyl)propionamide (3bMP1) inhibits the growth of M. tuberculosis, and resistance to this compound is conferred by mutation within a member of the proline-proline-glutamate (PPE) family, PPE51. Deletion of PPE51 rendered M. tuberculosis cells unable to replicate on propionamide, glucose, or glycerol. Growth was restored upon loss of the mycobacterial cell wall component phthiocerol dimycocerosate. Mutants in other proline-glutamate (PE)/PPE clusters, responsive to magnesium and phosphate, also showed a phthiocerol dimycocerosate-dependent growth compromise upon limitation of the corresponding substrate. Phthiocerol dimycocerosate determined the low permeability of the mycobacterial outer membrane, and the PE/PPE proteins apparently act as solute-specific channels. © 2020 American Association for the Advancement of Science. All rights reserved.
关键词	3,3 bis di(methylsulfonyl)propionamide antiinfective agent bacterial protein glucose glycerol magnesium phosphate propionamide derivative protein proline proline glutamate protein proline proline glutamate 51 unclassified drug amide bacterial protein lipid phthiocerol dimycocerosate propionamide thiol derivative cell component chemical compound membrane mutation protein tuberculosis antibiotic resistance Article bacterial cell bacterial cell wall bacterial growth bacterial outer membrane cell division drug structure Mycobacterium tuberculosis nonhuman nutrient uptake priority journal protein depletion cell membrane cell membrane permeability chemistry drug effect gene deletion genetics metabolism transport at the cellular level Mycobacterium tuberculosis Amides Bacterial Proteins Biological Transport Cell Membrane Cell Membrane Permeability Drug Resistance, Bacterial Gene Deletion Glucose Glycerol Lipids Mycobacterium tuberculosis Sulfhydryl Compounds
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133662
推荐引用方式 GB/T 7714	Wang Q.,Boshoff H.I.M.,Harrison J.R.,et al. PE/PPE proteins mediate nutrient transport across the outer membrane of Mycobacterium tuberculosis[J]. Science,2020,367(6482).
APA	Wang Q..,Boshoff H.I.M..,Harrison J.R..,Ray P.C..,Green S.R..,...&Barry Iii C.E..(2020).PE/PPE proteins mediate nutrient transport across the outer membrane of Mycobacterium tuberculosis.,367(6482).
MLA	Wang Q.,et al."PE/PPE proteins mediate nutrient transport across the outer membrane of Mycobacterium tuberculosis".367.6482(2020).