气候变化领域集成服务门户(CCPortal): Microenvironment mapping via Dexter energy transfer on immune cells

CCPortal

DOI	10.1126/science.aaz5074
	Microenvironment mapping via Dexter energy transfer on immune cells
	Geri J.B.; Oakley J.V.; Reyes-Robles T.; Wang T.; McCarver S.J.; White C.H.; Rodriguez-Rivera F.P.; Parker D.L.; Hett E.C.; Fadeyi O.O.; Oslund R.C.; MacMillan D.W.C.
发表日期	2020
ISSN	0036-8075
起始页码	1091
结束页码	1097
卷号	367 期号:6482
英文摘要	Many disease pathologies can be understood through the elucidation of localized biomolecular networks, or microenvironments. To this end, enzymatic proximity labeling platforms are broadly applied for mapping the wider spatial relationships in subcellular architectures. However, technologies that can map microenvironments with higher precision have long been sought. Here, we describe a microenvironment-mapping platform that exploits photocatalytic carbene generation to selectively identify protein-protein interactions on cell membranes, an approach we term MicroMap (mMap). By using a photocatalyst-antibody conjugate to spatially localize carbene generation, we demonstrate selective labeling of antibody binding targets and their microenvironment protein neighbors. This technique identified the constituent proteins of the programmed-death ligand 1 (PD-L1) microenvironment in live lymphocytes and selectively labeled within an immunosynaptic junction. © 2020 American Association for the Advancement of Science. All rights reserved.
关键词	antibody conjugate carbene programmed death 1 ligand 1 methane programmed death 1 ligand 1 antibody catalysis catalyst cell component detection method enzyme enzyme activity protein antigen binding Article cell membrane energy transfer human immunocompetent cell immunological synapse lymphocyte photocatalysis priority journal protein analysis protein protein interaction tumor microenvironment catalysis chemistry energy transfer Jurkat cell line metabolism photochemistry procedures radiation response ultraviolet radiation B7-H1 Antigen Catalysis Cell Membrane Cellular Microenvironment Energy Transfer Humans Jurkat Cells Lymphocytes Methane Photochemical Processes Protein Interaction Mapping Protein Interaction Maps Ultraviolet Rays
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133657
推荐引用方式 GB/T 7714	Geri J.B.,Oakley J.V.,Reyes-Robles T.,et al. Microenvironment mapping via Dexter energy transfer on immune cells[J]. Science,2020,367(6482).
APA	Geri J.B..,Oakley J.V..,Reyes-Robles T..,Wang T..,McCarver S.J..,...&MacMillan D.W.C..(2020).Microenvironment mapping via Dexter energy transfer on immune cells.,367(6482).
MLA	Geri J.B.,et al."Microenvironment mapping via Dexter energy transfer on immune cells".367.6482(2020).