气候变化领域集成服务门户(CCPortal): Pervasive functional translation of noncanonical human open reading frames

CCPortal

DOI	10.1126/science.aav5912
	Pervasive functional translation of noncanonical human open reading frames
	Chen J.; Brunner A.-D.; Cogan J.Z.; Nuñez J.K.; Fields A.P.; Adamson B.; Itzhak D.N.; Li J.Y.; Mann M.; Leonetti M.D.; Weissman J.S.
发表日期	2020
ISSN	0036-8075
起始页码	140
结束页码	146
卷号	367 期号:6482
英文摘要	Ribosome profiling has revealed pervasive but largely uncharacterized translation outside of canonical coding sequences (CDSs). In this work, we exploit a systematic CRISPR-based screening strategy to identify hundreds of noncanonical CDSs that are essential for cellular growth and whose disruption elicits specific, robust transcriptomic and phenotypic changes in human cells. Functional characterization of the encoded microproteins reveals distinct cellular localizations, specific protein binding partners, and hundreds of microproteins that are presented by the human leukocyte antigen system. We find multiple microproteins encoded in upstream open reading frames, which form stable complexes with the main, canonical protein encoded on the same messenger RNA, thereby revealing the use of functional bicistronic operons in mammals. Together, our results point to a family of functional human microproteins that play critical and diverse cellular roles. © 2020 American Association for the Advancement of Science. All rights reserved.
关键词	messenger RNA antigen cell component cell organelle chemical binding growth phenotype protein Quaternary RNA Article cell function cell growth cellular distribution clustered regularly interspaced short palindromic repeat gene sequence genetic screening HLA system human non-canonical base pairing open reading frame operon priority journal protein analysis protein binding protein function transcriptomics translation regulation Mammalia
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133649
推荐引用方式 GB/T 7714	Chen J.,Brunner A.-D.,Cogan J.Z.,et al. Pervasive functional translation of noncanonical human open reading frames[J]. Science,2020,367(6482).
APA	Chen J..,Brunner A.-D..,Cogan J.Z..,Nuñez J.K..,Fields A.P..,...&Weissman J.S..(2020).Pervasive functional translation of noncanonical human open reading frames.,367(6482).
MLA	Chen J.,et al."Pervasive functional translation of noncanonical human open reading frames".367.6482(2020).