气候变化领域集成服务门户(CCPortal): Cryo-EM structure of a neuronal functional amyloid implicated in memory persistence in Drosophila

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DOI	10.1126/science.aaw1114
	Cryo-EM structure of a neuronal functional amyloid implicated in memory persistence in Drosophila
	Hervas R.; Rau M.J.; Park Y.; Zhang W.; Murzin A.G.; Fitzpatrick J.A.J.; Scheres S.H.W.; Si K.
发表日期	2020
ISSN	0036-8075
起始页码	1230
结束页码	1234
卷号	367 期号:6483
英文摘要	How long-lived memories withstand molecular turnover is a fundamental question. Aggregates of a prion-like RNA-binding protein, cytoplasmic polyadenylation element-binding (CPEB) protein, is a putative substrate of long-lasting memories. We isolated aggregated Drosophila CPEB, Orb2, from adult heads and determined its activity and atomic structure, at 2.6-angstrom resolution, using cryo-electron microscopy. Orb2 formed ∼75-nanometer-long threefold-symmetric amyloid filaments. Filament formation transformed Orb2 from a translation repressor to an activator and "seed" for further translationally active aggregation. The 31-amino acid protofilament core adopted a cross-b unit with a single hydrophilic hairpin stabilized through interdigitated glutamine packing. Unlike the hydrophobic core of pathogenic amyloids, the hydrophilic core of Orb2 filaments suggests how some neuronal amyloids could be a stable yet regulatable substrate ofmemory. © 2020 American Association for the Advancement of Science. All rights reserved.
关键词	amyloid protein cytoplasmic polyadenylation element binding protein insect protein protein Orb2 unclassified drug adult memory neurology protein substrate turnover Article cryoelectron microscopy Drosophila enzyme activity hydrophilicity hydrophobicity memory nerve cell nonhuman priority journal protein aggregation protein function protein isolation protein processing protein structure
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133640
推荐引用方式 GB/T 7714	Hervas R.,Rau M.J.,Park Y.,et al. Cryo-EM structure of a neuronal functional amyloid implicated in memory persistence in Drosophila[J]. Science,2020,367(6483).
APA	Hervas R..,Rau M.J..,Park Y..,Zhang W..,Murzin A.G..,...&Si K..(2020).Cryo-EM structure of a neuronal functional amyloid implicated in memory persistence in Drosophila.,367(6483).
MLA	Hervas R.,et al."Cryo-EM structure of a neuronal functional amyloid implicated in memory persistence in Drosophila".367.6483(2020).