气候变化领域集成服务门户(CCPortal): Liquid-liquid phase separation drives skin barrier formation

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DOI	10.1126/science.aax9554
	Liquid-liquid phase separation drives skin barrier formation
	Quiroz F.G.; Fiore V.F.; Levorse J.; Polak L.; Wong E.; Pasolli H.A.; Fuchs E.
发表日期	2020
ISSN	10959203
卷号	367 期号:6483
英文摘要	At the body surface, skin's stratified squamous epithelium is challenged by environmental extremes. The surface of the skin is composed of enucleated, flattened surface squames. They derive from underlying, transcriptionally active keratinocytes that display filaggrin-containing keratohyalin granules (KGs) whose function is unclear. Here, we found that filaggrin assembles KGs through liquid-liquid phase separation. The dynamics of phase separation governed terminal differentiation and were disrupted by human skin barrier disease-associated mutations. We used fluorescent sensors to investigate endogenous phase behavior in mice. Phase transitions during epidermal stratification crowded cellular spaces with liquid-like KGs whose coalescence was restricted by keratin filament bundles. We imaged cells as they neared the skin surface and found that environmentally regulated KG phase dynamics drive squame formation. Thus, epidermal structure and function are driven by phase-separation dynamics. Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
关键词	filaggrin intermediate filament protein keratin keratohyalin animal cytoplasm epidermis genetics human keratinocyte metabolism mouse phase transition physiology Animals Cytoplasm Epidermis Humans Intermediate Filament Proteins Keratinocytes Keratins Mice Phase Transition
语种	英语
来源机构	Science (New York, N.Y.)
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133625
推荐引用方式 GB/T 7714	Quiroz F.G.,Fiore V.F.,Levorse J.,et al. Liquid-liquid phase separation drives skin barrier formation[J]. Science (New York, N.Y.),2020,367(6483).
APA	Quiroz F.G..,Fiore V.F..,Levorse J..,Polak L..,Wong E..,...&Fuchs E..(2020).Liquid-liquid phase separation drives skin barrier formation.,367(6483).
MLA	Quiroz F.G.,et al."Liquid-liquid phase separation drives skin barrier formation".367.6483(2020).