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| DOI | 10.1126/science.aaz9837 |
| Liquid-liquid phase separation drives skin barrier formation | |
| Quiroz F.G.; Fiore V.F.; Levorse J.; Polak L.; Wong E.; Pasolli H.A.; Fuchs E. | |
| 发表日期 | 2020 |
| ISSN | 0036-8075 |
| 卷号 | 367期号:6483 |
| 英文摘要 | At the body surface, skin's stratified squamous epithelium is challenged by environmental extremes. The surface of the skin is composed of enucleated, flattened surface squames. They derive from underlying, transcriptionally active keratinocytes that display filaggrin-containing keratohyalin granules (KGs) whose function is unclear. Here, we found that filaggrin assembles KGs through liquid-liquid phase separation. The dynamics of phase separation governed terminal differentiation and were disrupted by human skin barrier disease-associated mutations. We used fluorescent sensors to investigate endogenous phase behavior in mice. Phase transitions during epidermal stratification crowded cellular spaces with liquid-like KGs whose coalescence was restricted by keratin filament bundles. We imaged cells as they neared the skin surface and found that environmentally regulated KG phase dynamics drive squame formation. Thus, epidermal structure and function are driven by phase-separation dynamics. © 2020 American Association for the Advancement of Science. All rights reserved. |
| 关键词 | filaggrinkeratohyalincoalescencedifferentiationseparationskinskin disorderstratificationamino acid compositionArticlebioengineeringbiophysicscell differentiationcell functioncell structureepidermis cellgenetic transfectionhumankeratinocyteliquidlive cell imagingmolecular dynamicsnonhumanpHphase separationphase transitionpriority journalproteomicssequence analysissignal transductionskinskin surfaceMus |
| 语种 | 英语 |
| 来源机构 | Science |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/133624 |
| 推荐引用方式 GB/T 7714 | Quiroz F.G.,Fiore V.F.,Levorse J.,et al. Liquid-liquid phase separation drives skin barrier formation[J]. Science,2020,367(6483). |
| APA | Quiroz F.G..,Fiore V.F..,Levorse J..,Polak L..,Wong E..,...&Fuchs E..(2020).Liquid-liquid phase separation drives skin barrier formation.,367(6483). |
| MLA | Quiroz F.G.,et al."Liquid-liquid phase separation drives skin barrier formation".367.6483(2020). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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