气候变化领域集成服务门户(CCPortal): Genetically targeted chemical assembly of functional materials in living cells, tissues, and animals

CCPortal

DOI	10.1126/science.aay4866
	Genetically targeted chemical assembly of functional materials in living cells, tissues, and animals
	Liu J.; Kim Y.S.; Richardson C.E.; Tom A.; Ramakrishnan C.; Birey F.; Katsumata T.; Chen S.; Wang C.; Wang X.; Joubert L.-M.; Jiang Y.; Wang H.; Fenno L.E.; Tok J.B.-H.; Paşca S.P.; Shen K.; Bao Z.; Deisseroth K.
发表日期	2020
ISSN	0036-8075
起始页码	1372
结束页码	1376
卷号	367 期号:6484
英文摘要	The structural and functional complexity of multicellular biological systems, such as the brain, are beyond the reach of human design or assembly capabilities. Cells in living organisms may be recruited to construct synthetic materials or structures if treated as anatomically defined compartments for specific chemistry, harnessing biology for the assembly of complex functional structures. By integrating engineered-enzyme targeting and polymer chemistry, we genetically instructed specific living neurons to guide chemical synthesis of electrically functional (conductive or insulating) polymers at the plasma membrane. Electrophysiological and behavioral analyses confirmed that rationally designed, genetically targeted assembly of functional polymers not only preserved neuronal viability but also achieved remodeling of membrane properties and modulated cell type-specific behaviors in freely moving animals. This approach may enable the creation of diverse, complex, and functional structures and materials within living systems. © 2020 American Association for the Advancement of Science. All rights reserved.
关键词	poly(3,4 ethylenedioxythiophene)polyaniline polymer unclassified drug 3-nitro-o-phenylenediamine aniline aniline derivative ascorbate peroxidase nitro derivative phenylenediamine derivative polymer anatomy genetic engineering membrane physiological response polymer animal cell Article biocompatibility brain nerve cell brain slice Caenorhabditis elegans cell membrane cell viability controlled study genetic procedures genetically targeted chemical assemby hippocampal neuronal culture human human cell in vivo study nerve cell membrane nonhuman polymerization priority journal rat synthesis action potential animal cell culture cell survival chemistry electric conductivity enzymology genetic engineering genetic transduction genetics HEK293 cell line hippocampus membrane potential metabolism motoneuron mouse muscle cell nerve cell patch clamp technique physiology Animalia Action Potentials Aniline Compounds Animals Ascorbate Peroxidases Caenorhabditis elegans Cell Membrane Cell Survival Cells, Cultured Electric Conductivity Genetic Engineering HEK293 Cells Hippocampus Humans Membrane Potentials Mice Motor Neurons Muscle Cells Neurons Nitro Compounds Patch-Clamp Techniques Phenylenediamines Polymers Rats Transduction, Genetic
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133618
推荐引用方式 GB/T 7714	Liu J.,Kim Y.S.,Richardson C.E.,et al. Genetically targeted chemical assembly of functional materials in living cells, tissues, and animals[J]. Science,2020,367(6484).
APA	Liu J..,Kim Y.S..,Richardson C.E..,Tom A..,Ramakrishnan C..,...&Deisseroth K..(2020).Genetically targeted chemical assembly of functional materials in living cells, tissues, and animals.,367(6484).
MLA	Liu J.,et al."Genetically targeted chemical assembly of functional materials in living cells, tissues, and animals".367.6484(2020).