气候变化领域集成服务门户(CCPortal): Transcription polymerase-catalyzed emergence of novel RNA replicons

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DOI	10.1126/science.aay0688
	Transcription polymerase-catalyzed emergence of novel RNA replicons
	Jain N.; Blauch L.R.; Szymanski M.R.; Das R.; Tang S.K.Y.; Yin Y.W.; Fire A.Z.
发表日期	2020
ISSN	0036-8075
卷号	368 期号:6487
英文摘要	Transcription polymerases can exhibit an unusual mode of regenerating certain RNA templates from RNA, yielding systems that can replicate and evolve with RNA as the information carrier. Two classes of pathogenic RNAs (hepatitis delta virus in animals and viroids in plants) are copied by host transcription polymerases. Using in vitro RNA replication by the transcription polymerase of T7 bacteriophage as an experimental model, we identify hundreds of new replicating RNAs, define three mechanistic hallmarks of replication (subterminal de novo initiation, RNA shape-shifting, and interrupted rolling-circle synthesis), and describe emergence from DNA seeds as a mechanism for the origin of novel RNA replicons. These results inform models for the origins and replication of naturally occurring RNA genetic elements and suggest a means by which diverse RNA populations could be propagated as hereditary material in cellular contexts. © 2020 American Association for the Advancement of Science. All rights reserved.
关键词	cell component enzyme enzyme activity genetic analysis RNA article bacteriophage catalysis DNA replication origin heredity in vitro study nonhuman plant seed replicon RNA replication synthesis transcription initiation biocatalysis biosynthesis genetic transcription metabolism Animalia Hepatitis delta virus Viroids bacteriophage T7 RNA polymerase DNA directed RNA polymerase RNA viral protein Biocatalysis DNA-Directed RNA Polymerases Replicon RNA Transcription, Genetic Viral Proteins
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133576
推荐引用方式 GB/T 7714	Jain N.,Blauch L.R.,Szymanski M.R.,et al. Transcription polymerase-catalyzed emergence of novel RNA replicons[J]. Science,2020,368(6487).
APA	Jain N..,Blauch L.R..,Szymanski M.R..,Das R..,Tang S.K.Y..,...&Fire A.Z..(2020).Transcription polymerase-catalyzed emergence of novel RNA replicons.,368(6487).
MLA	Jain N.,et al."Transcription polymerase-catalyzed emergence of novel RNA replicons".368.6487(2020).