气候变化领域集成服务门户(CCPortal): Intravital imaging of mouse embryos

CCPortal

DOI	10.1126/science.aba0210
	Intravital imaging of mouse embryos
	Huang Q.; Cohen M.A.; Alsina F.C.; Devlin G.; Garrett A.; McKey J.; Havlik P.; Rakhilin N.; Wang E.; Xiang K.; Mathews P.; Wang L.; Bock C.; Ruthig V.; Wang Y.; Negrete M.; Wong C.W.; Murthy P.K.L.; Zhang S.; Daniel A.R.; Kirsch D.G.; Kang Y.; Capel B.; Asokan A.; Silver D.L.; Jaenisch R.; Shen X.
发表日期	2020
ISSN	0036-8075
起始页码	181
结束页码	186
卷号	368 期号:6487
英文摘要	Embryonic development is a complex process that is unamenable to direct observation. In this study, we implanted a window to the mouse uterus to visualize the developing embryo from embryonic day 9.5 to birth. This removable intravital window allowed manipulation and high-resolution imaging. In live mouse embryos, we observed transient neurotransmission and early vascularization of neural crest cell (NCC)-derived perivascular cells in the brain, autophagy in the retina, viral gene delivery, and chemical diffusion through the placenta. We combined the imaging window with in utero electroporation to label and track cell division and movement within embryos and observed that clusters of mouse NCC-derived cells expanded in interspecies chimeras, whereas adjacent human donor NCC-derived cells shrank. This technique can be combined with various tissue manipulation and microscopy methods to study the processes of development at unprecedented spatiotemporal resolution. © 2020 The Authors.
关键词	cell embryo embryonic development imaging method physiology animal cell animal experiment article autophagy (cellular)brain cell cell division chimera controlled study diffusion electroporation embryo female microscopy mouse mouse embryo neural crest cell neurotransmission nonhuman placenta retina uterus vascularization viral gene delivery system
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133574
推荐引用方式 GB/T 7714	Huang Q.,Cohen M.A.,Alsina F.C.,et al. Intravital imaging of mouse embryos[J]. Science,2020,368(6487).
APA	Huang Q..,Cohen M.A..,Alsina F.C..,Devlin G..,Garrett A..,...&Shen X..(2020).Intravital imaging of mouse embryos.,368(6487).
MLA	Huang Q.,et al."Intravital imaging of mouse embryos".368.6487(2020).