气候变化领域集成服务门户(CCPortal): The Ccr4-Not complex monitors the translating ribosome for codon optimality

CCPortal

DOI	10.1126/science.aay6912
	The Ccr4-Not complex monitors the translating ribosome for codon optimality
	Buschauer R.; Matsuo Y.; Sugiyama T.; Chen Y.-H.; Alhusaini N.; Sweet T.; Ikeuchi K.; Cheng J.; Matsuki Y.; Nobuta R.; Gilmozzi A.; Berninghausen O.; Tesina P.; Becker T.; Coller J.; Inada T.; Beckmann R.
发表日期	2020
ISSN	0036-8075
卷号	368 期号:6488
英文摘要	Control of messenger RNA (mRNA) decay rate is intimately connected to translation elongation, but the spatial coordination of these events is poorly understood. The Ccr4-Not complex initiates mRNA decay through deadenylation and activation of decapping. We used a combination of cryo-electron microscopy, ribosome profiling, and mRNA stability assays to examine the recruitment of Ccr4-Not to the ribosome via specific interaction of the Not5 subunit with the ribosomal E-site in Saccharomyces cerevisiae. This interaction occurred when the ribosome lacked accommodated A-site transfer RNA, indicative of low codon optimality. Loss of the interaction resulted in the inability of the mRNA degradation machinery to sense codon optimality. Our findings elucidate a physical link between the Ccr4-Not complex and the ribosome and provide mechanistic insight into the coupling of decoding efficiency with mRNA stability. © 2020 American Association for the Advancement of Science. All rights reserved.
关键词	exonuclease initiation factor 5A messenger RNA ribosome RNA transfer RNA ubiquitin protein ligase E3 bioassay biomonitoring cell component plant and fungus structure and form 40S ribosomal subunit adenylation amino terminal sequence Article binding site biochemical analysis carbon catabolite repressor 4 negative on tata complex codon complex formation controlled study cryoelectron microscopy homeostasis molecular interaction nonhuman priority journal protein conformation ribosome Saccharomyces cerevisiae sequence analysis ubiquitination Saccharomyces cerevisiae
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133559
推荐引用方式 GB/T 7714	Buschauer R.,Matsuo Y.,Sugiyama T.,et al. The Ccr4-Not complex monitors the translating ribosome for codon optimality[J]. Science,2020,368(6488).
APA	Buschauer R..,Matsuo Y..,Sugiyama T..,Chen Y.-H..,Alhusaini N..,...&Beckmann R..(2020).The Ccr4-Not complex monitors the translating ribosome for codon optimality.,368(6488).
MLA	Buschauer R.,et al."The Ccr4-Not complex monitors the translating ribosome for codon optimality".368.6488(2020).