气候变化领域集成服务门户(CCPortal): Structure of a trapped radical transfer pathway within a ribonucleotide reductase holocomplex

CCPortal

DOI	10.1126/science.aba6794
	Structure of a trapped radical transfer pathway within a ribonucleotide reductase holocomplex
	Kang G.; Taguchi A.T.; Stubbe J.; Drennan C.L.
发表日期	2020
ISSN	0036-8075
起始页码	424
结束页码	427
卷号	368 期号:6489
英文摘要	Ribonucleotide reductases (RNRs) are a diverse family of enzymes that are alone capable of generating 2′-deoxynucleotides de novo and are thus critical in DNA biosynthesis and repair. The nucleotide reduction reaction in all RNRs requires the generation of a transient active site thiyl radical, and in class I RNRs, this process involves a long-range radical transfer between two subunits, a and b. Because of the transient subunit association, an atomic resolution structure of an active a2b2 RNR complex has been elusive. We used a doubly substituted b2, E52Q/(2,3,5)-trifluorotyrosine122-b2, to trap wild-type a2 in a long-lived a2b2 complex. We report the structure of this complex by means of cryo–electron microscopy to 3.6-angstrom resolution, allowing for structural visualization of a 32-angstrom-long radical transfer pathway that affords RNR activity. Copyright © 2020 The Authors,
关键词	ribonucleotide reductase DNA enzyme enzyme activity radical Article beta sheet binding site catalysis cryoelectron microscopy enzyme active site enzyme activity enzyme binding enzyme conformation enzyme structure enzyme subunit nonhuman priority journal signal transduction
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133551
推荐引用方式 GB/T 7714	Kang G.,Taguchi A.T.,Stubbe J.,et al. Structure of a trapped radical transfer pathway within a ribonucleotide reductase holocomplex[J]. Science,2020,368(6489).
APA	Kang G.,Taguchi A.T.,Stubbe J.,&Drennan C.L..(2020).Structure of a trapped radical transfer pathway within a ribonucleotide reductase holocomplex.,368(6489).
MLA	Kang G.,et al."Structure of a trapped radical transfer pathway within a ribonucleotide reductase holocomplex".368.6489(2020).