气候变化领域集成服务门户(CCPortal): Light-powered CO2 fixation in a chloroplast mimic with natural and synthetic parts

CCPortal

DOI	10.1126/science.aaz6802
	Light-powered CO2 fixation in a chloroplast mimic with natural and synthetic parts
	Miller T.E.; Beneyton T.; Schwander T.; Diehl C.; Girault M.; McLean R.; Chotel T.; Claus P.; Cortina N.S.; Baret J.-C.; Erb T.J.
发表日期	2020
ISSN	0036-8075
起始页码	649
结束页码	654
卷号	368 期号:6491
英文摘要	Nature integrates complex biosynthetic and energy-converting tasks within compartments such as chloroplasts and mitochondria. Chloroplasts convert light into chemical energy, driving carbon dioxide fixation. We used microfluidics to develop a chloroplast mimic by encapsulating and operating photosynthetic membranes in cell-sized droplets. These droplets can be energized by light to power enzymes or enzyme cascades and analyzed for their catalytic properties in multiplex and real time. We demonstrate how these microdroplets can be programmed and controlled by adjusting internal compositions and by using light as an external trigger. We showcase the capability of our platform by integrating the crotonyl–coenzyme A (CoA)/ethylmalonyl-CoA/hydroxybutyryl-CoA (CETCH) cycle, a synthetic network for carbon dioxide conversion, to create an artificial photosynthetic system that interfaces the natural and the synthetic biological worlds. Copyright © 2020 The Authors, some rights reserved;
关键词	carbon dioxide coenzyme A crotonyl coenzyme A ethylmalonyl coenzyme A hydroxybutyryl coenzyme A unclassified drug acyl coenzyme A carbon dioxide ethylmalonyl-coenzyme A carbon dioxide carbon fixation catalysis catalyst chloroplast enzyme enzyme activity Article automation cell size chemical analysis chloroplast controlled study encapsulation enzyme activity enzyme mechanism microfluidics photoreactivity photosynthesis physical parameters priority journal biocatalysis biomimetics carbon cycle chloroplast light metabolism photosynthesis radiation response spinach Acyl Coenzyme A Biocatalysis Biomimetics Carbon Cycle Carbon Dioxide Chloroplasts Light Photosynthesis Spinacia oleracea
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133515
推荐引用方式 GB/T 7714	Miller T.E.,Beneyton T.,Schwander T.,et al. Light-powered CO2 fixation in a chloroplast mimic with natural and synthetic parts[J]. Science,2020,368(6491).
APA	Miller T.E..,Beneyton T..,Schwander T..,Diehl C..,Girault M..,...&Erb T.J..(2020).Light-powered CO2 fixation in a chloroplast mimic with natural and synthetic parts.,368(6491).
MLA	Miller T.E.,et al."Light-powered CO2 fixation in a chloroplast mimic with natural and synthetic parts".368.6491(2020).