气候变化领域集成服务门户(CCPortal): Synthesis of proteins by automated flow chemistry

CCPortal

DOI	10.1126/science.abb2491
	Synthesis of proteins by automated flow chemistry
	Hartrampf N.; Saebi A.; Poskus M.; Gates Z.P.; Callahan A.J.; Cowfer A.E.; Hanna S.; Antilla S.; Schissel C.K.; Quartararo A.J.; Ye X.; Mijalis A.J.; Simon M.D.; Loas A.; Liu S.; Jessen C.; Nielsen T.E.; Pentelute B.L.
发表日期	2020
ISSN	0036-8075
起始页码	980
结束页码	987
卷号	368 期号:6494
英文摘要	Ribosomes can produce proteins in minutes and are largely constrained to proteinogenic amino acids. Here, we report highly efficient chemistry matched with an automated fast-flow instrument for the direct manufacturing of peptide chains up to 164 amino acids long over 327 consecutive reactions. The machine is rapid: Peptide chain elongation is complete in hours. We demonstrate the utility of this approach by the chemical synthesis of nine different protein chains that represent enzymes, structural units, and regulatory factors. After purification and folding, the synthetic materials display biophysical and enzymatic properties comparable to the biologically expressed proteins. High-fidelity automated flow chemistry is an alternative for producing single-domain proteins without the ribosome. © 2020 American Association for the Advancement of Science. All rights reserved.
关键词	amino acid cell component chemistry instrumentation peptide protein purification peptide protein chemistry isolation and purification procedures protein domain protein folding solid phase synthesis synthesis Peptides Protein Domains Protein Folding Proteins Solid-Phase Synthesis Techniques
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133464
推荐引用方式 GB/T 7714	Hartrampf N.,Saebi A.,Poskus M.,et al. Synthesis of proteins by automated flow chemistry[J]. Science,2020,368(6494).
APA	Hartrampf N..,Saebi A..,Poskus M..,Gates Z.P..,Callahan A.J..,...&Pentelute B.L..(2020).Synthesis of proteins by automated flow chemistry.,368(6494).
MLA	Hartrampf N.,et al."Synthesis of proteins by automated flow chemistry".368.6494(2020).