气候变化领域集成服务门户(CCPortal): Barcoded microbial system for high-resolution object provenance

CCPortal

DOI	10.1126/science.aba5584
	Barcoded microbial system for high-resolution object provenance
	Qian J.; Lu Z.-X.; Mancuso C.P.; Jhuang H.-Y.; Del Carmen Barajas-Ornelas R.; Boswell S.A.; Ramírez-Guadiana F.H.; Jones V.; Sonti A.; Sedlack K.; Artzi L.; Jung G.; Arammash M.; Pettit M.E.; Melfi M.; Lyon L.; Owen S.V.; Baym M.; Khalil A.S.; Silver P.A.; Rudner D.Z.; Springer M.
发表日期	2020
ISSN	10959203
起始页码	1135
结束页码	1140
卷号	368 期号:6495
英文摘要	Determining where an object has been is a fundamental challenge for human health, commerce, and food safety. Location-specific microbes in principle offer a cheap and sensitive way to determine object provenance. We created a synthetic, scalable microbial spore system that identifies object provenance in under 1 hour at meter-scale resolution and near single-spore sensitivity and can be safely introduced into and recovered from the environment. This system solves the key challenges in object provenance: persistence in the environment, scalability, rapid and facile decoding, and biocontainment. Our system is compatible with SHERLOCK, a Cas13a RNA-guided nucleic acid detection assay, facilitating its implementation in a wide range of applications. Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
关键词	article food safety human microorganism nonhuman nucleic acid analysis
语种	英语
来源机构	Science (New York, N.Y.)
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133458
推荐引用方式 GB/T 7714	Qian J.,Lu Z.-X.,Mancuso C.P.,et al. Barcoded microbial system for high-resolution object provenance[J]. Science (New York, N.Y.),2020,368(6495).
APA	Qian J..,Lu Z.-X..,Mancuso C.P..,Jhuang H.-Y..,Del Carmen Barajas-Ornelas R..,...&Springer M..(2020).Barcoded microbial system for high-resolution object provenance.,368(6495).
MLA	Qian J.,et al."Barcoded microbial system for high-resolution object provenance".368.6495(2020).