气候变化领域集成服务门户(CCPortal): The structure of human CST reveals a decameric assembly bound to telomeric DNA

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DOI	10.1126/science.aaz9649
	The structure of human CST reveals a decameric assembly bound to telomeric DNA
	Lim C.J.; Barbour A.T.; Zaug A.J.; Goodrich K.J.; McKay A.E.; Wuttke D.S.; Cech T.R.
发表日期	2020
ISSN	0036-8075
起始页码	1081
结束页码	1085
卷号	368 期号:6495
英文摘要	The CTC1-STN1-TEN1 (CST) complex is essential for telomere maintenance and resolution of stalled replication forks genome-wide. Here, we report the 3.0-angstrom cryo-electron microscopy structure of human CST bound to telomeric single-stranded DNA (ssDNA), which assembles as a decameric supercomplex. The atomic model of the 134-kilodalton CTC1 subunit, built almost entirely de novo, reveals the overall architecture of CST and the DNA-binding anchor site. The carboxyl-terminal domain of STN1 interacts with CTC1 at two separate docking sites, allowing allosteric mediation of CST decamer assembly. Furthermore, ssDNA appears to staple two monomers to nucleate decamer assembly. CTC1 has stronger structural similarity to Replication Protein A than the expected similarity to yeast Cdc13. The decameric structure suggests that CST can organize ssDNA analogously to the nucleosome's organization of double-stranded DNA. © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works
关键词	carboxylic acid chemical binding DNA genome microscopy protein yeast
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133452
推荐引用方式 GB/T 7714	Lim C.J.,Barbour A.T.,Zaug A.J.,et al. The structure of human CST reveals a decameric assembly bound to telomeric DNA[J]. Science,2020,368(6495).
APA	Lim C.J..,Barbour A.T..,Zaug A.J..,Goodrich K.J..,McKay A.E..,...&Cech T.R..(2020).The structure of human CST reveals a decameric assembly bound to telomeric DNA.,368(6495).
MLA	Lim C.J.,et al."The structure of human CST reveals a decameric assembly bound to telomeric DNA".368.6495(2020).