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DOI10.1126/science.aaz9649
The structure of human CST reveals a decameric assembly bound to telomeric DNA
Lim C.J.; Barbour A.T.; Zaug A.J.; Goodrich K.J.; McKay A.E.; Wuttke D.S.; Cech T.R.
发表日期2020
ISSN0036-8075
起始页码1081
结束页码1085
卷号368期号:6495
英文摘要The CTC1-STN1-TEN1 (CST) complex is essential for telomere maintenance and resolution of stalled replication forks genome-wide. Here, we report the 3.0-angstrom cryo-electron microscopy structure of human CST bound to telomeric single-stranded DNA (ssDNA), which assembles as a decameric supercomplex. The atomic model of the 134-kilodalton CTC1 subunit, built almost entirely de novo, reveals the overall architecture of CST and the DNA-binding anchor site. The carboxyl-terminal domain of STN1 interacts with CTC1 at two separate docking sites, allowing allosteric mediation of CST decamer assembly. Furthermore, ssDNA appears to staple two monomers to nucleate decamer assembly. CTC1 has stronger structural similarity to Replication Protein A than the expected similarity to yeast Cdc13. The decameric structure suggests that CST can organize ssDNA analogously to the nucleosome's organization of double-stranded DNA. © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works
关键词carboxylic acidchemical bindingDNAgenomemicroscopyproteinyeast
语种英语
来源机构Science
文献类型期刊论文
条目标识符http://gcip.llas.ac.cn/handle/2XKMVOVA/133452
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GB/T 7714
Lim C.J.,Barbour A.T.,Zaug A.J.,et al. The structure of human CST reveals a decameric assembly bound to telomeric DNA[J]. Science,2020,368(6495).
APA Lim C.J..,Barbour A.T..,Zaug A.J..,Goodrich K.J..,McKay A.E..,...&Cech T.R..(2020).The structure of human CST reveals a decameric assembly bound to telomeric DNA.,368(6495).
MLA Lim C.J.,et al."The structure of human CST reveals a decameric assembly bound to telomeric DNA".368.6495(2020).
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