Climate Change Data Portal
| DOI | 10.1126/science.aay7794 |
| Membrane-proximal F-actin restricts local membrane protrusions and directs cell migration | |
| Bisaria A.; Hayer A.; Garbett D.; Cohen D.; Meyer T. | |
| 发表日期 | 2020 |
| ISSN | 0036-8075 |
| 起始页码 | 1205 |
| 结束页码 | 1210 |
| 卷号 | 368期号:6496 |
| 英文摘要 | Cell migration is driven by local membrane protrusion through directed polymerization of F-actin at the front. However, F-actin next to the plasma membrane also tethers the membrane and thus resists outgoing protrusions. Here, we developed a fluorescent reporter to monitor changes in the density of membrane-proximal F-actin (MPA) during membrane protrusion and cell migration. Unlike the total F-actin concentration, which was high in the front of migrating cells, MPA density was low in the front and high in the back. Back-to-front MPA density gradients were controlled by higher cofilin-mediated turnover of F-actin in the front. Furthermore, nascent membrane protrusions selectively extended outward from areas where MPA density was reduced. Thus, locally low MPA density directs local membrane protrusions and stabilizes cell polarization during cell migration. © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works |
| 关键词 | cell componentconcentration (composition)membranepolymerizationproteinactingreen fluorescent proteincell membranecell motioncell polaritycell surfacechemistrygeneticshumanmetabolismumbilical vein endothelial cellActinsCell MembraneCell MovementCell PolarityCell Surface ExtensionsGreen Fluorescent ProteinsHuman Umbilical Vein Endothelial CellsHumans |
| 语种 | 英语 |
| 来源机构 | Science |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/133428 |
| 推荐引用方式 GB/T 7714 | Bisaria A.,Hayer A.,Garbett D.,et al. Membrane-proximal F-actin restricts local membrane protrusions and directs cell migration[J]. Science,2020,368(6496). |
| APA | Bisaria A.,Hayer A.,Garbett D.,Cohen D.,&Meyer T..(2020).Membrane-proximal F-actin restricts local membrane protrusions and directs cell migration.,368(6496). |
| MLA | Bisaria A.,et al."Membrane-proximal F-actin restricts local membrane protrusions and directs cell migration".368.6496(2020). |
| 条目包含的文件 | 条目无相关文件。 | |||||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
