气候变化领域集成服务门户(CCPortal): Structural evidence for a dynamic metallocofactor during N2 reduction by Mo-nitrogenase

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DOI	10.1126/science.aaz6748
	Structural evidence for a dynamic metallocofactor during N2 reduction by Mo-nitrogenase
	Kang W.; Lee C.C.; Jasniewski A.J.; Ribbe M.W.; Hu Y.
发表日期	2020
ISSN	0036-8075
起始页码	1381
结束页码	1385
卷号	368 期号:6497
英文摘要	The enzyme nitrogenase uses a suite of complex metallocofactors to reduce dinitrogen (N2) to ammonia. Mechanistic details of this reaction remain sparse. We report a 1.83-angstrom crystal structure of the nitrogenase molybdenum-iron (MoFe) protein captured under physiological N2 turnover conditions. This structure reveals asymmetric displacements of the cofactor belt sulfurs (S2B or S3A and S5A) with distinct dinitrogen species in the two ab dimers of the protein. The sulfur-displaced sites are distinct in the ability of protein ligands to donate protons to the bound dinitrogen species, as well as the elongation of either the Mo-O5 (carboxyl) or Mo-O7 (hydroxyl) distance that switches the Mo-homocitrate ligation from bidentate to monodentate. These results highlight the dynamic nature of the cofactor during catalysis and provide evidence for participation of all belt-sulfur sites in this process. © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works
关键词	ammonia catalysis crystal structure enzyme activity ligand physiological response sulfur ligand molybdenum iron protein nitrogen sulfur Azotobacter vinelandii biocatalysis chemistry enzymology oxidation reduction reaction protein multimerization X ray crystallography Azotobacter vinelandii Biocatalysis Crystallography, X-Ray Ligands Molybdoferredoxin Nitrogen Oxidation-Reduction Protein Multimerization Sulfur
语种	英语
来源机构	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133410
推荐引用方式 GB/T 7714	Kang W.,Lee C.C.,Jasniewski A.J.,et al. Structural evidence for a dynamic metallocofactor during N2 reduction by Mo-nitrogenase[J]. Science,2020,368(6497).
APA	Kang W.,Lee C.C.,Jasniewski A.J.,Ribbe M.W.,&Hu Y..(2020).Structural evidence for a dynamic metallocofactor during N2 reduction by Mo-nitrogenase.,368(6497).
MLA	Kang W.,et al."Structural evidence for a dynamic metallocofactor during N2 reduction by Mo-nitrogenase".368.6497(2020).