气候变化领域集成服务门户(CCPortal): Mechanisms of OCT4-SOX2 motif readout on nucleosomes

CCPortal

DOI	10.1126/science.abb0074
	Mechanisms of OCT4-SOX2 motif readout on nucleosomes
	Michael A.K.; Grand R.S.; Isbel L.; Cavadini S.; Kozicka Z.; Kempf G.; Bunker R.D.; Schenk A.D.; Graff-Meyer A.; Pathare G.R.; Weiss J.; Matsumoto S.; Burger L.; Schübeler D.; Thomä N.H.
发表日期	2020
ISSN	10959203
起始页码	1460
结束页码	1465
卷号	368 期号:6498
英文摘要	Transcription factors (TFs) regulate gene expression through chromatin where nucleosomes restrict DNA access. To study how TFs bind nucleosome-occupied motifs, we focused on the reprogramming factors OCT4 and SOX2 in mouse embryonic stem cells. We determined TF engagement throughout a nucleosome at base-pair resolution in vitro, enabling structure determination by cryo-electron microscopy at two preferred positions. Depending on motif location, OCT4 and SOX2 differentially distort nucleosomal DNA. At one position, OCT4-SOX2 removes DNA from histone H2A and histone H3; however, at an inverted motif, the TFs only induce local DNA distortions. OCT4 uses one of its two DNA-binding domains to engage DNA in both structures, reading out a partial motif. These findings explain site-specific nucleosome engagement by the pluripotency factors OCT4 and SOX2, and they reveal how TFs distort nucleosomes to access chromatinized motifs. Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
语种	英语
来源机构	Science (New York, N.Y.)
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133401
推荐引用方式 GB/T 7714	Michael A.K.,Grand R.S.,Isbel L.,et al. Mechanisms of OCT4-SOX2 motif readout on nucleosomes[J]. Science (New York, N.Y.),2020,368(6498).
APA	Michael A.K..,Grand R.S..,Isbel L..,Cavadini S..,Kozicka Z..,...&Thomä N.H..(2020).Mechanisms of OCT4-SOX2 motif readout on nucleosomes.,368(6498).
MLA	Michael A.K.,et al."Mechanisms of OCT4-SOX2 motif readout on nucleosomes".368.6498(2020).