气候变化领域集成服务门户(CCPortal): Site-specific glycan analysis of the SARS-CoV-2 spike

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DOI	10.1126/science.abb9983
	Site-specific glycan analysis of the SARS-CoV-2 spike
	Watanabe Y.; Allen J.D.; Wrapp D.; McLellan J.S.; Crispin M.
发表日期	2020
ISSN	10959203
起始页码	330
结束页码	333
卷号	369 期号:6501
英文摘要	The emergence of the betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), represents a considerable threat to global human health. Vaccine development is focused on the principal target of the humoral immune response, the spike (S) glycoprotein, which mediates cell entry and membrane fusion. The SARS-CoV-2 S gene encodes 22 N-linked glycan sequons per protomer, which likely play a role in protein folding and immune evasion. Here, using a site-specific mass spectrometric approach, we reveal the glycan structures on a recombinant SARS-CoV-2 S immunogen. This analysis enables mapping of the glycan-processing states across the trimeric viral spike. We show how SARS-CoV-2 S glycans differ from typical host glycan processing, which may have implications in viral pathobiology and vaccine design. Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
语种	英语
来源机构	Science (New York, N.Y.)
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133342
推荐引用方式 GB/T 7714	Watanabe Y.,Allen J.D.,Wrapp D.,et al. Site-specific glycan analysis of the SARS-CoV-2 spike[J]. Science (New York, N.Y.),2020,369(6501).
APA	Watanabe Y.,Allen J.D.,Wrapp D.,McLellan J.S.,&Crispin M..(2020).Site-specific glycan analysis of the SARS-CoV-2 spike.,369(6501).
MLA	Watanabe Y.,et al."Site-specific glycan analysis of the SARS-CoV-2 spike".369.6501(2020).