气候变化领域集成服务门户(CCPortal): CRISPR-CasΦ from huge phages is a hypercompact genome editor

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DOI	10.1126/science.abb1400
	CRISPR-CasΦ from huge phages is a hypercompact genome editor
	Pausch P.; Al-Shayeb B.; Bisom-Rapp E.; Tsuchida C.A.; Li Z.; Cress B.F.; Knott G.J.; Jacobsen S.E.; Banfield J.F.; Doudna J.A.
发表日期	2020
ISSN	10959203
起始页码	333
结束页码	337
卷号	369 期号:6501
英文摘要	CRISPR-Cas systems are found widely in prokaryotes, where they provide adaptive immunity against virus infection and plasmid transformation. We describe a minimal functional CRISPR-Cas system, comprising a single ~70-kilodalton protein, CasΦ, and a CRISPR array, encoded exclusively in the genomes of huge bacteriophages. CasΦ uses a single active site for both CRISPR RNA (crRNA) processing and crRNA-guided DNA cutting to target foreign nucleic acids. This hypercompact system is active in vitro and in human and plant cells with expanded target recognition capabilities relative to other CRISPR-Cas proteins. Useful for genome editing and DNA detection but with a molecular weight half that of Cas9 and Cas12a genome-editing enzymes, CasΦ offers advantages for cellular delivery that expand the genome editing toolbox. Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
语种	英语
来源机构	Science (New York, N.Y.)
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133318
推荐引用方式 GB/T 7714	Pausch P.,Al-Shayeb B.,Bisom-Rapp E.,et al. CRISPR-CasΦ from huge phages is a hypercompact genome editor[J]. Science (New York, N.Y.),2020,369(6501).
APA	Pausch P..,Al-Shayeb B..,Bisom-Rapp E..,Tsuchida C.A..,Li Z..,...&Doudna J.A..(2020).CRISPR-CasΦ from huge phages is a hypercompact genome editor.,369(6501).
MLA	Pausch P.,et al."CRISPR-CasΦ from huge phages is a hypercompact genome editor".369.6501(2020).