气候变化领域集成服务门户(CCPortal): Diversity decoupled from sulfur isotope fractionation in a sulfate-reducing microbial community

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DOI	10.1111/gbi.12356
	Diversity decoupled from sulfur isotope fractionation in a sulfate-reducing microbial community
	Colangelo-Lillis J.; Pelikan C.; Herbold C.W.; Altshuler I.; Loy A.; Whyte L.G.; Wing B.A.
发表日期	2019
ISSN	14724677
起始页码	660
结束页码	675
卷号	17 期号:6
英文摘要	The extent of fractionation of sulfur isotopes by sulfate-reducing microbes is dictated by genomic and environmental factors. A greater understanding of species-specific fractionations may better inform interpretation of sulfur isotopes preserved in the rock record. To examine whether gene diversity influences net isotopic fractionation in situ, we assessed environmental chemistry, sulfate reduction rates, diversity of putative sulfur-metabolizing organisms by 16S rRNA and dissimilatory sulfite reductase (dsrB) gene amplicon sequencing, and net fractionation of sulfur isotopes along a sediment transect of a hypersaline Arctic spring. In situ sulfate reduction rates yielded minimum cell-specific sulfate reduction rates < 0.3 × 10−15 moles cell−1 day−1. Neither 16S rRNA nor dsrB diversity indices correlated with relatively constant (38‰–45‰) net isotope fractionation (ε34Ssulfide-sulfate). Measured ε34S values could be reproduced in a mechanistic fractionation model if 1%–2% of the microbial community (10%–60% of Deltaproteobacteria) were engaged in sulfate respiration, indicating heterogeneous respiratory activity within sulfate-reducing populations. This model indicated enzymatic kinetic diversity of Apr was more likely to correlate with sulfur fractionation than DsrB. We propose that, above a threshold Shannon diversity value of 0.8 for dsrB, the influence of the specific composition of the microbial community responsible for generating an isotope signal is overprinted by the control exerted by environmental variables on microbial physiology. © 2019 John Wiley & Sons Ltd
关键词	biomarker environmental factor enzyme enzyme activity geodiversity isotopic fractionation microbial activity microbial community species diversity sulfate-reducing bacterium sulfur isotope Arctic Deltaproteobacteria sulfate sulfur bacterium classification metabolism Mexico microbiology microflora oxidation reduction reaction pond Bacteria Mexico Microbiota Oxidation-Reduction Ponds Sulfates Sulfur Isotopes
语种	英语
来源机构	Geobiology
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/133223
推荐引用方式 GB/T 7714	Colangelo-Lillis J.,Pelikan C.,Herbold C.W.,et al. Diversity decoupled from sulfur isotope fractionation in a sulfate-reducing microbial community[J]. Geobiology,2019,17(6).
APA	Colangelo-Lillis J..,Pelikan C..,Herbold C.W..,Altshuler I..,Loy A..,...&Wing B.A..(2019).Diversity decoupled from sulfur isotope fractionation in a sulfate-reducing microbial community.,17(6).
MLA	Colangelo-Lillis J.,et al."Diversity decoupled from sulfur isotope fractionation in a sulfate-reducing microbial community".17.6(2019).