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DOI	10.1111/1744-7917.12608
	Identification and functional characterization of doublesex gene in the testis of Spodoptera litura
	Du, Qian; Wen, Liang; Zheng, Si-Chun; Bi, Hong-Lun; Huang, Yong-Ping; Feng, Qi-Li; Liu, Lin
发表日期	2019
ISSN	1672-9609
EISSN	1744-7917
卷号	26 期号:6
英文摘要	Fusion of the testis occurs in most Lepidoptera insects, including Spodoptera litura, an important polyphagous pest. Testicular fusion in S. litura is advantageous for male reproduction, and the molecular mechanism of fusion remains unknown. Doublesex influences the formation of genitalia, the behavior of courtship, and sexually dimorphic traits in fruit-fly and silkworm, and is essential for sexual differentiation. However, its purpose in the testis of S. litura remains unknown. The doublesex gene of S. litura (Sldsx) has male-specific Sldsx(M) and female-specific Sldsx(F) isoforms, and exhibits a higher expression level in the male testis. At the testicular fusion stage (L6D6), Sldsx attained the highest expression compared to the pre-fusion and post-fusion periods. Moreover, Sldsx had a higher expression in the peritoneal sheaths of testis than that of germ cells in the follicle. CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/Cas9) was applied to S. litura to determine the role of Sldsx. A mixture of single guide RNA messenger RNA and Cas9 protein (300 ng/mu L each) was injected into eggs within 2 h following oviposition. CRISPR/Cas9 successfully induced genomic mutagenesis of Sldsx at G(0) generation. The mutant males had smaller testis surrounded by less tracheae. Moreover, the mutant males had abnormal external genitalia and could not finish mating with wild-type females. Additionally, testes were fused for almost all mutant males. The results showed that Sldsx was not related to testicular fusion, and is required for both testis development and the formation and function of external genitalia in S. litura. The main roles of doublesex on the male are similar to other insects.
关键词	CRISPR Cas9 system doublesex external genitalia Spodoptera litura testis
学科领域	Entomology
语种	英语
WOS研究方向	Entomology
来源期刊	INSECT SCIENCE
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/112068
作者单位	South China Normal Univ, Sch Life Sci, Inst Insect Sci & Technol, Guangzhou Key Lab Insect Dev Regulat & Appl Res, Guangzhou 510631, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Du, Qian,Wen, Liang,Zheng, Si-Chun,et al. Identification and functional characterization of doublesex gene in the testis of Spodoptera litura[J]. 中国科学院西北生态环境资源研究院,2019,26(6).
APA	Du, Qian.,Wen, Liang.,Zheng, Si-Chun.,Bi, Hong-Lun.,Huang, Yong-Ping.,...&Liu, Lin.(2019).Identification and functional characterization of doublesex gene in the testis of Spodoptera litura.INSECT SCIENCE,26(6).
MLA	Du, Qian,et al."Identification and functional characterization of doublesex gene in the testis of Spodoptera litura".INSECT SCIENCE 26.6(2019).