气候变化领域集成服务门户(CCPortal): Gene duplication confers enhanced expression of 27-kDa gamma-zein for endosperm modification in quality protein maize

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DOI	10.1073/pnas.1601352113
	Gene duplication confers enhanced expression of 27-kDa gamma-zein for endosperm modification in quality protein maize
	Liu, Hongjun; Shi, Junpeng; Sun, Chuanlong; Gong, Hao; Fan, Xingming; Qiu, Fazhan; Huang, Xuehui; Feng, Qi; Zheng, Xixi; Yuan, Ningning; Li, Changsheng; Zhang, Zhiyong; Deng, Yiting; Wang, Jiechen; Pan, Guangtang; Han, Bin; Lai, Jinsheng; Wu, Yongrui
发表日期	2016
ISSN	0027-8424
卷号	113 期号:18
英文摘要	The maize opaque2 (o2) mutant has a high nutritional value but it develops a chalky endosperm that limits its practical use. Genetic selection for o2 modifiers can convert the normally chalky endosperm of the mutant into a hard, vitreous phenotype, yielding what is known as quality protein maize (QPM). Previous studies have shown that enhanced expression of 27-kDa gamma-zein in QPM is essential for endosperm modification. Taking advantage of genome-wide association study analysis of a natural population, linkage mapping analysis of a recombinant inbred line population, and map-based cloning, we identified a quantitative trait locus (q gamma 27) affecting expression of 27-kDa gamma-zein. q gamma 27 was mapped to the same region as the major o2 modifier (o2 modifier1) on chromosome 7 near the 27-kDa gamma-zein locus. q gamma 27 resulted from a 15.26-kb duplication at the 27-kDa gamma-zein locus, which increases the level of gene expression. This duplication occurred before maize domestication; however, the gene structure of q gamma 27 appears to be unstable and the DNA rearrangement frequently occurs at this locus. Because enhanced expression of 27-kDa gamma-zein is critical for endosperm modification in QPM, q gamma 27 is expected to be under artificial selection. This discovery provides a useful molecular marker that can be used to accelerate QPM breeding.
关键词	QPM o2 modifiers endosperm artificial selection gene duplication
学科领域	Science & Technology - Other Topics
语种	英语
WOS研究方向	Multidisciplinary Sciences
来源期刊	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/111881
作者单位	Chinese Acad Sci, Shanghai Inst Biol Sci, Natl Key Lab Plant Mol Genet, Ctr Excellence Mol Plant Sci,Inst Plant Physiol &, Shanghai 200032, Peoples R China
推荐引用方式 GB/T 7714	Liu, Hongjun,Shi, Junpeng,Sun, Chuanlong,et al. Gene duplication confers enhanced expression of 27-kDa gamma-zein for endosperm modification in quality protein maize[J]. 中国科学院西北生态环境资源研究院,2016,113(18).
APA	Liu, Hongjun.,Shi, Junpeng.,Sun, Chuanlong.,Gong, Hao.,Fan, Xingming.,...&Wu, Yongrui.(2016).Gene duplication confers enhanced expression of 27-kDa gamma-zein for endosperm modification in quality protein maize.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,113(18).
MLA	Liu, Hongjun,et al."Gene duplication confers enhanced expression of 27-kDa gamma-zein for endosperm modification in quality protein maize".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 113.18(2016).