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DOI | 10.1016/j.jgg.2011.11.004 |
Development and high-throughput genotyping of substitution lines carring the chromosome segments of indica 9311 in the background of japonica Nipponbare | |
Zhang, Hua; Zhao, Qiang; Sun, Zhi-Zhong; Zhang, Chang-Quan; Feng, Qi![]() | |
发表日期 | 2011 |
ISSN | 1673-8527 |
EISSN | 1873-5533 |
卷号 | 38期号:12 |
英文摘要 | Chromosome segment substitution lines (CSSLs) are useful for the precise mapping of quantitative trait loci (QTLs) and dissection of the genetic basis of complex traits. In this study, two whole-genome sequenced rice cultivars, the japonica Nipponbare and indica 9311 were used as recipient and donor, respectively. A population with 57 CSSLs was developed after crossing and back-crossing assisted by molecular markers, and genotypes were identified using a high-throughput resequencing strategy. Detailed graphical genotypes of 38 lines were constructed based on resequencing data. These CSSLs had a total of 95 substituted segments derived from indica 9311, with an average of about 2.5 segments per CSSL and eight segments per chromosome, and covered about 87.4% of the rice whole genome. A multiple linear regression QTL analysis mapped four QTLs for 1000-grain weight. The largest-effect QTL was located in a region on chromosome 5 that contained a cloned major QTL GW5/qSW5 for grain size in rice. These CSSLs with a background of Nipponbare may provide powerful tools for future whole-genome discovery and functional study of essential genes/QTLs in rice, and offer ideal materials and foundations for japonica breeding. |
关键词 | Oryza sativa L.Chromosome segment substitution lines (CSSLs)Molecular marker-assisted selectionHigh-throughput resequencing |
学科领域 | Biochemistry & Molecular Biology; Genetics & Heredity |
语种 | 英语 |
WOS研究方向 | Biochemistry & Molecular Biology ; Genetics & Heredity |
来源期刊 | JOURNAL OF GENETICS AND GENOMICS
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来源机构 | 中国科学院西北生态环境资源研究院 |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/111630 |
作者单位 | Yangzhou Univ, Jiangsu Key Lab Crop Genet & Physiol, Minist Educ Plant Funct Genom, Key Lab,Coll Agr, Yangzhou 225009, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Hua,Zhao, Qiang,Sun, Zhi-Zhong,et al. Development and high-throughput genotyping of substitution lines carring the chromosome segments of indica 9311 in the background of japonica Nipponbare[J]. 中国科学院西北生态环境资源研究院,2011,38(12). |
APA | Zhang, Hua.,Zhao, Qiang.,Sun, Zhi-Zhong.,Zhang, Chang-Quan.,Feng, Qi.,...&Liu, Qiao-Quan.(2011).Development and high-throughput genotyping of substitution lines carring the chromosome segments of indica 9311 in the background of japonica Nipponbare.JOURNAL OF GENETICS AND GENOMICS,38(12). |
MLA | Zhang, Hua,et al."Development and high-throughput genotyping of substitution lines carring the chromosome segments of indica 9311 in the background of japonica Nipponbare".JOURNAL OF GENETICS AND GENOMICS 38.12(2011). |
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