气候变化领域集成服务门户(CCPortal): Development and high-throughput genotyping of substitution lines carring the chromosome segments of indica 9311 in the background of japonica Nipponbare

CCPortal

DOI	10.1016/j.jgg.2011.11.004
	Development and high-throughput genotyping of substitution lines carring the chromosome segments of indica 9311 in the background of japonica Nipponbare
	Zhang, Hua; Zhao, Qiang; Sun, Zhi-Zhong; Zhang, Chang-Quan; Feng, Qi; Tang, Shu-Zhu; Liang, Guo-Hua; Gu, Ming-Hong; Han, Bin; Liu, Qiao-Quan
发表日期	2011
ISSN	1673-8527
EISSN	1873-5533
卷号	38 期号:12
英文摘要	Chromosome segment substitution lines (CSSLs) are useful for the precise mapping of quantitative trait loci (QTLs) and dissection of the genetic basis of complex traits. In this study, two whole-genome sequenced rice cultivars, the japonica Nipponbare and indica 9311 were used as recipient and donor, respectively. A population with 57 CSSLs was developed after crossing and back-crossing assisted by molecular markers, and genotypes were identified using a high-throughput resequencing strategy. Detailed graphical genotypes of 38 lines were constructed based on resequencing data. These CSSLs had a total of 95 substituted segments derived from indica 9311, with an average of about 2.5 segments per CSSL and eight segments per chromosome, and covered about 87.4% of the rice whole genome. A multiple linear regression QTL analysis mapped four QTLs for 1000-grain weight. The largest-effect QTL was located in a region on chromosome 5 that contained a cloned major QTL GW5/qSW5 for grain size in rice. These CSSLs with a background of Nipponbare may provide powerful tools for future whole-genome discovery and functional study of essential genes/QTLs in rice, and offer ideal materials and foundations for japonica breeding.
关键词	Oryza sativa L.Chromosome segment substitution lines (CSSLs)Molecular marker-assisted selection High-throughput resequencing
学科领域	Biochemistry & Molecular Biology; Genetics & Heredity
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
来源期刊	JOURNAL OF GENETICS AND GENOMICS
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/111630
作者单位	Yangzhou Univ, Jiangsu Key Lab Crop Genet & Physiol, Minist Educ Plant Funct Genom, Key Lab,Coll Agr, Yangzhou 225009, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Hua,Zhao, Qiang,Sun, Zhi-Zhong,et al. Development and high-throughput genotyping of substitution lines carring the chromosome segments of indica 9311 in the background of japonica Nipponbare[J]. 中国科学院西北生态环境资源研究院,2011,38(12).
APA	Zhang, Hua.,Zhao, Qiang.,Sun, Zhi-Zhong.,Zhang, Chang-Quan.,Feng, Qi.,...&Liu, Qiao-Quan.(2011).Development and high-throughput genotyping of substitution lines carring the chromosome segments of indica 9311 in the background of japonica Nipponbare.JOURNAL OF GENETICS AND GENOMICS,38(12).
MLA	Zhang, Hua,et al."Development and high-throughput genotyping of substitution lines carring the chromosome segments of indica 9311 in the background of japonica Nipponbare".JOURNAL OF GENETICS AND GENOMICS 38.12(2011).